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Database searching in mass spectrometry based proteomics
Current Bioinformatics. 2012. Vol. 7. No. 2. P. 221–230.
Кертес-Фаркаш А., Myers M. P.
Bottom-up proteomics (mass spectrometry analysis of peptides obtained by proteolysis and separated by liquid chromatography, (LCMS/MS)) is one of the most frequently used techniques for identifying and characterizing proteins in biological samples. A key element of the analysis is database searching when the mass spectra of the peptides are compared with a database of theoretically computed (or experimental) peptide spectra. Here we discuss the main computational approaches to spectrum database searching and the statistical analysis of the results.
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