?
Heterologous production of antimicrobial peptides in yeast allows for massive assessment of the activity of DNA-encoded antimicrobials in situ
Acta Naturae. 2025. Vol. 17. No. 1. P. 71–77.
Pipiya S. O., Ivanova A. O., Mokrushina Y. A., I. E. Eliseev, A. G. Gabibov, I. V. Smirnov, Terekhov S. S.
Antibiotic resistance threatens global healthcare. In clinical practice, conventional antibiotics are becoming gradually less effective. Moreover, the introduction of new antimicrobial agents into clinical practice leads to the emergence of resistant pathogenic strains within just a few years. Hence, the development of platforms for massive creation and screening of new antimicrobial agents is of particular importance. Massive parallel screening will greatly reduce the time required to identify the most promising drug candidates. Meanwhile, DNA-encoded antimicrobial agents offer unique opportunities for the high-throughput development of new antibiotics. Here, the yeast Pichia pastoris was engineered to produce a panel of antimicrobial peptides (AMPs), followed by high-throughput screening of AMP producers that inhibit bacterial growth in situ. Yeast clones producing thanatin and protegrin-1 exhibited the highest level of antimicrobial activity among the panel of AMPs under investigation. The production level of recombinant thanatin was significantly higher than that of protegrin-1, which correlates with its low toxicity. The designed technique of massive assessment of the activity of DNA-encoded antimicrobial agents enables the identification of drug candidates with an increased therapeutic index. Further development of methods for a rational design of artificial diversity in AMPs, followed by deep functional profiling of antimicrobial activity, will yield new AMPs with improved therapeutic characteristics.
Language:
English
Gureev M., Advanced Science 2026 P. 1–13
Targeted protein degradation represents a promising therapeutic strategy, yet its broader application is often limited by the scarcity of usable E3 ligases. Glutathione peroxidase 4 (GPX4) is a key target for inducing ferroptosis, but achieving sustained and potent inhibition remains challenging with conventional enzymatic inhibitors. Herein, we report the first small‐molecule GPX4 degraders that incorporate ...
Added: September 26, 2026
Diachkova E., Nikulin D., Кубкин Я. В. et al., American Journal of Respiratory and Critical Care Medicine 2026 Vol. 212
Accurate classification of a patient with pulmonary hypertension (PH) into one of five groups is fundamental for appropriate disease management strategies. The time from disease onset to correct diagnosis is unacceptably long in many cases. Chronic thromboembolic pulmonary hypertension (CTEPH) develops as a consequence of venous thromboemboli, resulting in a more pronounced hereditary predisposition to ...
Added: September 23, 2026
Xu Y., Ryabov F., Alexandrov I. et al., USA 2026 Vol. 6 No. 8 Article 101324
Центромеры обеспечивают расхождение хромосом, однако организацию их хроматина в пределах повторяющейся альфа-сателлитной ДНК долгое время было трудно изучать. Для решения этой задачи мы создали гаплотип-разрешённые аннотации сателлитной ДНК для полной диплоидной сборки генома человека T2T-HG002. Затем с помощью метода направленного метилирования с секвенированием длинных прочтений (DiMeLo-seq) мы картировали центромерный белок A (CENP-A), модификацию H3K9me3 и ...
Added: September 23, 2026
Hebbar P., Ryabov F., Alexandrov I. et al., Cell 2026 Vol. 189 P. 4891–4908.e12
The common marmoset is a New World monkey widely used to study primate evolution and human disease. We present a telomere-to-telomere (T2T) reference assembly for the species, plus three near-T2T haplotypes. These resolve previously inaccessible regions, including the centromeres, sex chromosomes, subterminal satellites, acrocentric chromosomes, and the major histocompatibility complex (MHC). We find marmoset centromeres ...
Added: September 23, 2026
Shaitan A., Ke Y., Shutao C. et al., 2026 No. 24 Article 181
Background Therapeutic peptides exert pivotal effects in diverse biological processes, and have attracted significant interest in the field of biomedicine in recent years. However, most existing methods often fail to adequately capture the intricate interactions among amino acid residues and the contextual dependencies within peptide sequences, which hampers the extraction of deep semantic representations and ...
Added: September 18, 2026
Anton Rogachev, Sysoeva O., Frontiers in Systems Neuroscience 2025 Vol. 19 Article 1658243
Added: September 17, 2026
Rogachev A.O., Sysoeva O. V., Cognitive Systems Research 2024 Vol. 86 Article 101236
Receptive speech is the ability to understand speech addressed to a person. It is a crucial process for a child’s cognitive development. We examine the relationship between receptive speech and neural tracking of natural speech in 52 children aged 3–8 years to infer the neurophysiological mechanisms underlying speech development. We registered a 32-channel electroencephalogram (EEG) ...
Added: September 17, 2026
Poddiakov A., / Series Social Science Research Network "Social Science Research Network". 2026. No. 7437658.
Clarity of knowledge and reasoning is necessary in many cases. Yet vagueness in scientific thinking related to surprise, curiosity, "ability to engage with not-knowing" (de Freitas) and abductive reasoning is also a crucially important source of scientific creativity which supplements combinatorial logic when dealing with the already known. Starting from studies by C. S. Peirce ...
Added: September 15, 2026
Ребрейкина А. Б., Liaukovich K., Современная зарубежная психология 2024 Т. 13 № 2 С. 33–43
Создание методик диагностики на основе анализа движений глаз является особенно актуальным для оценки когнитивных функций у детей, которые не могут дать речевой или моторный ответ в силу своего возраста или из-за нарушений развития. Для таких детей использование объективных методов оценки когнитивных функций на основе айтрекинга может стать полезным диагностическим инструментом, дополняющим традиционные поведенческие опросники и ...
Added: September 8, 2026
Ребрейкина А. Б., Liaukovich K., Современная зарубежная психология 2023 Т. 12 № 4 С. 51–61
Активное развитие метода видеоокулографии, т. е. регистрации движений глаз, создает возможности для создания новых подходов для развития когнитивных функций и обучения. Методики, основанные на видеоокулографии, могут быть полезны не только для детей с серьезными нарушениями моторных функций, но и детей с нарушениями развития когнитивных функций, прежде всего внимания, так как контроль движений глаз тесно связан ...
Added: September 8, 2026
Портнова Г. В., Liaukovich K., Васильева Л. Н. et al., Журнал высшей нервной деятельности им. И.П. Павлова 2022 Т. 72 № 4 С. 504–519
Когнитивная и эмоциональная нагрузка в ходе увеличения сложности задач приводит к активации различных звеньев вегетативной нервной системы и может сопровождаться как увеличением эффективности решения задач, так и способствовать дестабилизации эмоционального состояния и уменьшению продуктивности. Увеличение когнитивной нагрузки при условии высокой мотивации испытуемых является стрессовым фактором и выражается в различной реакции симпатического и парасимпатического звеньев в ...
Added: September 8, 2026
Shaitan A., Ke Y., Hongjun Y. et al., PLoS Computational Biology 2026 Article e1013836
The interaction between peptides and human leukocyte antigen class II (HLA-II)
molecules plays a pivotal role in adaptive immune responses, as HLA-II mediates
the recognition of exogenous antigens and initiates T cell activation through peptide
presentation. Accurate prediction of peptide-HLA-II binding serves as a cornerstone
for deciphering cellular immune responses, and is essential for guiding the optimization
of antibody therapeutics. ...
Added: September 7, 2026
Proskurnina E., Liaukovich K., Bychkovskaya L. et al., Metabolites 2023 Vol. 13 No. 1 P. 73
Added: September 7, 2026
Liaukovich K., Sazhin S., Bobrov P. et al., International Journal of Molecular Sciences 2022 Vol. 23 No. 19 P. 11785
Added: September 7, 2026
Liaukovich K., Ukraintseva Y., Martynova O., Neuropsychologia 2022 Vol. 165 P. 108129
Added: September 7, 2026
Alshanskaia E., Portnova G., Liaukovich K. et al., Frontiers in Neuroscience 2024 Vol. 18
Added: September 7, 2026
Pipiya S., Mirzoeva N., Baranova M. et al., Acta Naturae 2023 Vol. 15 No. 1 P. 74–80
Bacterial infections caused by antibiotic-resistant pathogens pose an extremely serious and elusive problem in healthcare. The discovery and targeted creation of new antibiotics are today among the most important public health issues. Antibiotics based on antimicrobial peptides (AMPs) are of particular interest due to their genetically encoded nature. A distinct advantage of most AMPs is ...
Added: January 20, 2025
Eliseev I., Terterov I., Yudenko A. et al., Bioinformatics 2019 Vol. 35 No. 16 P. 2713–2717
Motivation: The rational design of antimicrobial peptides (AMPs) with increased therapeutic potential requires deep understanding of the determinants of their activities. Inspired by the computational linguistic approach, we hypothesized that sequence patterns may encode the functional features of AMPs.
Results: We found that α-helical and β-sheet peptides have non-intersecting pattern sets and therefore constructed new sequence ...
Added: January 20, 2025
Krenev I., Egorova E., Khaydukova M. et al., Biochemistry. Biokhimiia 2024 Vol. 89 No. 11 P. 2069–2082
The C3f peptide is a by-product of regulation of the activated complement system with no firmly established function of its own. We have previously shown that C3f exhibits moderate antimicrobial activity against some Gram-positive bacteria in vitro. Presence of two histidine residues in the amino acid sequence of the peptide suggests enhancement of its antimicrobial activity at ...
Added: January 20, 2025
Smetanin R., Sukhareva M., Vladimirova E. et al., Biochemical and Biophysical Research Communications 2024 Vol. 712-713 Article 149913
Innate immunity of invertebrates offers potent antimicrobial peptides (AMPs) against drug-resistant infections. To identify new worm β-hairpin AMPs, we explored the sequence diversity of proteins with a BRICHOS domain, which comprises worm AMP precursors. Strikingly, we discovered new BRICHOS AMPs not in worms, but in caecilians, the least studied clade of vertebrates. Two precursor proteins from Microcaecilia unicolorand Rhinatrema bivittatum resemble SP-C lung surfactants and bear ...
Added: January 19, 2025
Panina I., Balandin S., Tsarev A. et al., International Journal of Molecular Sciences 2023 Vol. 24 No. 2 P. 1
To date, a number of lantibiotics have been shown to use lipid II — a highly conserved peptidoglycan precursor in the cytoplasmic membrane of bacteria — as their molecular target. The α-component (Lchα) of the two-component lantibiotic lichenicidin (Lchα), previously isolated from the Bacillus licheniformis VK21 strain, seems to contain two putative lipid II binding ...
Added: February 21, 2023
Lohan S., Konshina A. G., Efremov R. et al., Journal of Medicinal Chemistry 2023 Vol. 66 No. 1 P. 855–874
A series of small (7- to 12-mer) amphipathic cationic peptides were designed and synthesized with the ultimate goal of creating a set of short helical peptides with broad-range bactericidal activity and good selectivity toward the bacterial cells. Activity analysis identified a lead 12-mer peptide 8b with broad spectrum activity against Gram-positive (MIC=3.1-6.2 µg/mL) and Gram-negative ...
Added: February 21, 2023
Lohan S., Mandal D., Choi W. et al., Journal of Medicinal Chemistry 2022 Vol. 65 No. 1 P. 665–687
We report the synthesis and antibacterial activities of a series of amphiphilic membrane-active peptides composed, in part, of various non-genetically coded hydrophobic amino acids. Lead cyclic peptides, 8C and 9C, showed broad-spectrum activity against drug-resistant Gram-positive (MIC=1.5-6.2 µg/mL) and Gram-negative (MIC=12.5-25 µg/mL) bacteria. Cytotoxicity study showed the predominant lethal action of the peptides against bacteria ...
Added: February 1, 2022
Vladimir A. Korshun, Amino Acids 2018 Vol. 50 No. 12 P. 1697–1705
4-Chloro-l-kynurenine (3-(4-chloroanthraniloyl)-l-alanine, l-4-ClKyn), an amino acid known as a prospective antidepressant, was recently for the first time found in nature in the lipopeptide antibiotic taromycin. Here, we report another instance of its identification in a natural product: 4-chloro-l-kynurenine was isolated from acidic hydrolysis of a new complex peptide antibiotic INA-5812. l-4-ClKyn is a fluorescent compound responsible for the ...
Added: November 8, 2019