Metabolomics and viability of fibroblasts on the influence of the components of the malate dehydrogenase shuttle system in vitro

Сибирское медицинское обозрение. 2023. No. 1. P. 46 –50.

Kolotyeva N., Limareva L., Remizov V., Gilmiyarova n., Gusyakova O., E. A. Ryskina

The aim of the research.The aim is to evaluate the metabolic profile and viability of human dermal fibroblasts under the influence of malate and oxaloacetate in vitro.
Material and methods. Fibroblasts grown by the method of primary explants were incubated with solutions of malate and oxaloacetate. After that, the bio-
chemical profile and enzyme activity relative to intact fibroblasts in the cell culture supernatant and lysate were determined. Cell viability was determined
using an methyltetrazolium test (MTT-test). Statistical processing of the obtained research results was carried out in the environment of IBM SPSS Statistics
21 application programs.
Results. After incubation of fibroblasts with malate solution, the level of glucose, pyruvate and cholesterol in the supravascular fluid; pyruvate, glycerophos-
phate dehydrogenase and gamma-glutamyltranspeptidase in the cell lysate significantly changed. The effect of oxaloacetate is confirmed by a significant
change in the content of pyruvate and cholesterol in the cell culture supernatant; pyruvate, glycerophosphate dehydrogenase and gamma-glutamyltranspepti-
dase in fibroblast lysate. The use of the MTT-test revealed an increase in the viability of fibroblasts by 27,31% (p=0,019) and 33,83% (p=0,028) when growing
in an environment with malate and oxaloacetate, respectively.
Conclusion. The data obtained by us indicate that the components of the malate dehydrogenase shuttle can play a regulatory role, rearrange the metabolomics
of fibroblasts and have a protective effect on cell viability.

Keywords: Biochemistry, биохимия