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Изоформы микроРНК hsa-miR‐143-3p как регуляторы HK2 и SERPINE1 при гипоксии трофобласта и преэклампсии
Доклады российской академии наук. Науки о жизни. 2026. Т. 529. № 1. С. 389–395.
Preeclampsia (PE) is a severe obstetric complication caused by chronic placental ischaemia. Genome-wide profiling of microRNA isoforms in two trophoblast hypoxia models (CoCl2 and the oxyquinoline derivative neuradapt, BeWo b30 cells) and clinical PE samples (placental tissue, syncytiotrophoblast vesicles) revealed concordant downregulation of hsa-miR-143-3p isoforms. Under a strict fold-change threshold, the canonical isoform (21 nt) and uridylated miR-143-3p|0|−1(+1U) (21 nt) were downregulated across all conditions, and applying padj < 0.05 alone additionally identified miR-143-3p|0|+1 (22 nt) and miR-143-3p|0|0(+1U) (22 nt). Upregulation of direct targets SERPINE1 and HK2 and glycolytic genes LDHA, GAPDH, and PKM was observed in all conditions. TUT4 overexpression combined with DICER1 downregulation indicates systemic dysregulation of microRNA biogenesis. Downregulation of miR-143-3p isoforms represents a universal hypoxic signal in trophoblasts driving glycolytic reprogramming in PE.
Keywords: микроРНКгипоксияПреэклампсияhypoxiamicroRNAspre-eclampsiahsa-miR-143-3pSERPINE1hexokinase-2hsa-miR‐143-3pSERPINE1гексокиназа‐2
Publication based on the results of:
Knyazev E., Чеботарева О. Е., М.: Издательский дом НИУ ВШЭ, 2026.
This teaching and learning guide provides a concise overview of the theoretical and practical aspects of the basic skills required to work in a biological laboratory. It also includes protocols for practical classes conducted as part of the introductory professional training placement for first-year students in the Bachelor’s programme in Cell and Molecular Biotechnology at ...
Added: September 30, 2026
Ryskina H., Knyazev E., Вопросы биологической, медицинской и фармацевтической химии 2026 Т. 29 № 9 С. 84–91
Introduction. The active integration of rapidly developing omics and computer technologies in the 21st century has led to a breakthrough in understanding the principles of the functioning of living systems. One of the dynamically developing integrative areas in biology is biological modeling. The fundamental paradigm has been the methodology that allows for the prediction of ...
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Gureev M., Advanced Science 2026 P. 1–13
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Diachkova E., Nikulin D., Кубкин Я. В. et al., American Journal of Respiratory and Critical Care Medicine 2026 Vol. 212
Accurate classification of a patient with pulmonary hypertension (PH) into one of five groups is fundamental for appropriate disease management strategies. The time from disease onset to correct diagnosis is unacceptably long in many cases. Chronic thromboembolic pulmonary hypertension (CTEPH) develops as a consequence of venous thromboemboli, resulting in a more pronounced hereditary predisposition to ...
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Shaitan A., Ke Y., Shutao C. et al., 2026 No. 24 Article 181
Background Therapeutic peptides exert pivotal effects in diverse biological processes, and have attracted significant interest in the field of biomedicine in recent years. However, most existing methods often fail to adequately capture the intricate interactions among amino acid residues and the contextual dependencies within peptide sequences, which hampers the extraction of deep semantic representations and ...
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Anton Rogachev, Sysoeva O., Cognitive Systems Research 2024 Vol. 86 Article 101236
Receptive speech is the ability to understand speech addressed to a person. It is a crucial process for a child’s cognitive development. We examine the relationship between receptive speech and neural tracking of natural speech in 52 children aged 3–8 years to infer the neurophysiological mechanisms underlying speech development. We registered a 32-channel electroencephalogram (EEG) ...
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Ребрейкина А. Б., Liaukovich K., Современная зарубежная психология 2024 Т. 13 № 2 С. 33–43
Создание методик диагностики на основе анализа движений глаз является особенно актуальным для оценки когнитивных функций у детей, которые не могут дать речевой или моторный ответ в силу своего возраста или из-за нарушений развития. Для таких детей использование объективных методов оценки когнитивных функций на основе айтрекинга может стать полезным диагностическим инструментом, дополняющим традиционные поведенческие опросники и ...
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Ребрейкина А. Б., Liaukovich K., Современная зарубежная психология 2023 Т. 12 № 4 С. 51–61
Активное развитие метода видеоокулографии, т. е. регистрации движений глаз, создает возможности для создания новых подходов для развития когнитивных функций и обучения. Методики, основанные на видеоокулографии, могут быть полезны не только для детей с серьезными нарушениями моторных функций, но и детей с нарушениями развития когнитивных функций, прежде всего внимания, так как контроль движений глаз тесно связан ...
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Humans and laboratory animals differ in their resistance to hypoxia, which affects the severity of inflammatory diseases and the rate of tumor progression. Therefore, it is necessary to search for methods for assessing the initial tolerance to oxygen deficiency and the risks of developing acute mountain sickness without direct hypoxia exposure on the organism. The ...
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Ок. Е. Колодеева, Ол. Е. Колодеева, Биотехнология 2025 Т. 41 № 6 С. 64–71
A three-dimensional (3D) matrix consisting of collagen fibers can create culture conditions that are more similar to in vivo tissues than 2D cultures in in vitro culture plates. Macrophages are among the most important innate immune cells. They are extremely plastic, allowing them to respond effectively to pro- and anti-inflammatory signals within their microenvironment. There ...
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Knyazev E., Timur Kulagin, Antipenko I. et al., Placenta 2026 Vol. 176 P. 1–12
Background. Preeclampsia (PE) complicates 2–8% of pregnancies and involves placental hypoxia and HIF-pathway activation, especially in early-onset PE (eoPE). Chemical mimetics like cobalt(II) chloride (CoCl2) and oxyquinoline derivatives model trophoblast hypoxia in vitro, yet their fidelity in recapitulating PE gene profiles remains unclear. Integrating patient tissue analyses with experimental models may reveal common markers and ...
Added: December 22, 2025
Antipenko I., Knyazev E., Timur Kulagin et al., Frontiers in Molecular Biosciences 2025 Vol. 12 Article 1731126
Preeclampsia is a leading cause of maternal and perinatal morbidity associated with systemic lipid metabolism disturbances, yet the underlying molecular mechanisms remain incompletely understood. In this study, we integrated single-cell RNA-seq data from preeclamptic placentas with an in vitro hypoxia model to analyze gene expression changes across distinct trophoblast subpopulations. While all trophoblast lineages exhibited ...
Added: December 2, 2025
Вишнякова П. А., Киселева В. В., Полтавец А. С. et al., Вестник Российского университета дружбы народов. Серия: Медицина 2025 Т. 29 № 3 С. 335–352
Relevance. Preeclampsia is a severe complication of pregnancy, occurring in 8% of cases and causing high maternal and perinatal mortality and morbidity. The etiology of preeclampsia is still a subject of research, however, more and more data indicate the involvement of innate immune cells, monocytes, in the pathogenesis of this dangerous pathology. The aim of ...
Added: September 12, 2025