Длительное культивирование макрофагов в коллагеновом геле на мембране (Transwell) индуцирует гипоксию

Биотехнология. 2025. Т. 41. № 6. С. 64–71.

Ок. Е. Колодеева, Ол. Е. Колодеева

A three-dimensional (3D) matrix consisting of collagen fibers can create culture conditions that are more similar to in vivo tissues than 2D cultures in in vitro culture plates. Macrophages are among the most important innate immune cells. They are extremely plastic, allowing them to respond effectively to pro- and anti-inflammatory signals within their microenvironment. There is growing evidence that macrophages in different tissues exhibit specific phenotypes, levels of activation, and functions. Therefore, models for studying the immune response should be close to physiological conditions, ensuring relevant tissue density. Culturing macrophages in 3D collagen matrices significantly alters cytokine secretion and gene expression. A protocol for the cultivation and differentiation of the THP-1 monocyte cell line into pro-inflammatory M1 macrophages in collagen gels of varying densities is proposed. It has been shown that macrophages in gel are capable of secreting pro-inflammatory cytokines, and prolonged cultivation induces hypoxia.

Keywords: гипоксия, hypoxia, M1 macrophages, type I collagen, THP-1 monocytes, proinflammatory cytokines, коллаген I типа, моноциты THP-1, макрофаги М1, провоспалительные цитокины