The results of the analysis of current and emerging technological shifts in Agro-Industrial Complex (AIC) at the global and national levels are presented in the article. The authors discuss the future of the Russian AIC through the prism of unlikely catastrophic events (jokers). The risks of those events should be taken into consideration when forming the agrarian and scientific-technical policy of the country by modeling stressful scenarios that can be realized in the domestic food sector as a result of triggering of certain drivers.
Real-time RT-PCR currently remains a gold standard for early COVID-19 diagnostics. However Loop-mediated isothermal amplification (LAMP) method outperform real-time RT-PCR in simplicity because it doesn't require expensive lab equipment and trained personnel, and in speed. LAMP-based diagnostic kits for COVID-19 testing are already exist, but LAMP-based tests have not been widely accepted yet. The method has great potential for massive diagnostics. Here we discuss technical and methodological aspects of its wide implementation.
Loop-mediated isothermal amplification (LAMP) of nucleic acids enables detection of amplification products in 10‒20 min after the reaction begins. An intraoperative method for detection of metastases in lymph nodes is based on LAMP and is called one-step nucleic acid amplification (OSNA). It allows rapid detection of the epithelial-specific marker KRT19 in lymph nodes. The standard protocol for OSNA was developed more than 10 years ago. Since that, a new version of the key enzyme involved in the reaction (Bst-polymerase) was obtained, but its use in OSNA remains unexplored. Moreover, the time has come to apply OSNA to the detection of other cancer types, in particular prostate cancer. The first step is to create an in vitro system that allows LAMP to be carried out on prostate cancer cell lines. Here, LAMP protocol for the new Bst 3.0 polymerase with optimized dNTPs concentration and without reverse transcriptase was developed, and cell lines (DU-145 for prostate cancer and Molt-4 for negative control) were selected. In summary, a new in vitro system for LAMP-based detection of prostate cancer marker KRT19 was developed.