Литографическая нанотехнология для создания устройств снабжения организма лекарственными препаратами
Inflammatory breast cancer (IBC) is an extremely malignant form of breast cancer which can be easily misdiagnosed. Conclusive prognostic IBC molecular biomarkers which are also providing the perspectives for targeted therapy are lacking so far. The aim of this study was to reveal the IBC-specific miRNA expression profile and to evaluate its association with clinicopathological parameters.
miRNA expression profiles of 13 IBC and 17 non-IBC patients were characterized using comprehensive Affymetrix GeneChip miRNA 3.0 microarray platform. Bioinformatic analysis was used to reveal IBC-specific miRNAs, deregulated pathways and potential miRNA targets.
31 differentially expressed miRNAs characterize IBC and mRNAs regulated by them and their associated pathways can functionally be attributed to IBC progression. In addition, a minimal predictive set of 4 miRNAs characteristic for the IBC phenotype and associated with the TP53 mutational status in breast cancer patients was identified.
We have characterized the complete miRNome of inflammatory breast cancer and found differentially expressed miRNAs which reliably classify the patients to IBC and non-IBC groups. We found that the mRNAs and pathways likely regulated by these miRNAs are highly relevant to cancer progression. Furthermore a minimal IBC-related predictive set of 4 miRNAs associated with the TP53 mutational status and survival for breast cancer patients was identified.
Nanotechnology allows scientists, engineers and physicians to move to large-scale research in the field of biology and public health at the cellular level and the molecular level. These studies will lead to new biotechnological production processes, as well as to the fundamental changes in the methods of medicine.  The use of the device forming the topological structure of a microchip on the substrate allows for the possibility to create a thumbnail template (using methods to increase the resolution of the projection lithography is proposed to obtain the elements of design rules with no more than 32 nm), the wavelength of ultraviolet radiation λ 193 nm. An example is the lead device "supply" of the body of drugs at the right time (Fig. 2). The device is a self-contained, miniature (solid-state silicon chip), implantable mechanism able to allocate according to a program contained in it is a substance (or substances). Clearly, such a mechanism may serve other functions (diagnostics, chemical analysis, etc.).  In the past few years, scientists have developed a technology rapid mapping of genetic information in DNA and RNA molecules, including the identification of mutations and expression levels. This technology uses a matrix of DNA microarrays, which is similar to the lithographic patterning technology for the industrial production of integrated circuits . Currently, these types of technologies become commercial importance and are used in biotechnology research and production processes. Development of new types of chemical matrices will expand the capabilities of these technologies and apply them in biological information processing apparatus or for analysis of proteins and other biomolecules. Miniaturization of devices based on related analytical processes, including electrophoresis, the reach of such technology and reduce the cost of many important analytical techniques, such as DNA sequencing or a fingerprint.