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Detection of Low-Abundant MicroRNAs with Hybridization Microchips
Bulletin of Experimental Biology and Medicine. 2019. Vol. 166. No. 6. P. 788–792.
The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips.
Language:
English
Poddiakov A., / Series Social Science Research Network "Social Science Research Network". 2026. No. 7437658.
Clarity of knowledge and reasoning is necessary in many cases. Yet vagueness in scientific thinking related to surprise, curiosity, "ability to engage with not-knowing" (de Freitas) and abductive reasoning is also a crucially important source of scientific creativity which supplements combinatorial logic when dealing with the already known. Starting from studies by C. S. Peirce ...
Added: September 15, 2026
Салагай О. О., Сахарова Г. М., Антонов Н. С. et al., Здоровье населения и среда обитания 2026 Т. 34 № 6 С. 7-17
Introduction: Tobacco and nicotine use is a risk factor for noncommunicable diseases, malignant neoplasms, and a severe course of infectious diseases. This factor, however, is manageable and can be modified through prevention programs allowing for trends and specifics of the use of tobacco and nicotine products.
Objective: To analyze patterns of tobacco and nicotine product use among the ...
Added: September 15, 2026
Салагай О. О., Сахарова Г. М., Антонов Н. С. et al., Медицинский вестник Юга России 2026 Т. 17 № 2 С. 109–119
Objective: analysis of the structure and characteristics of the consumption of various types of tobacco and nicotine-containing products by Veterans.
Materials and methods: the analysis was carried out based on the results of a survey of Veterans using a developed questionnaire. The survey was conducted at the offices of the Defenders of the Fatherland Foundation. The proportions ...
Added: September 15, 2026
Dimertsev A. V., Batmanova Y. V., Zhikhar T. V. et al., Burdenko's Journal of Neurosurgery, Россия 2025 Vol. 6 P. 38–45
Background. There are some unresolved issues in surgery for gliomas of Broca’s area (optimal intraoperative testing, cortical mapping, and stimulation parameters).
Objective. To clarify the peculiarities of neurosurgical technique and speech testing in surgery for gliomas of Broca’s area.
Material and methods. A single-center cohort retrospective study was devoted to surgical treatment of patients with Broca’s area ...
Added: September 14, 2026
Zheltyakova M., Korotkov A., Bursov K. et al., Herald of the Russian Academy of Sciences 2026 Vol. 95 P. 1048–1057
The Reading the Mind in the Eyes Test (RMET) is a widely used tool for assessing affective mentalization in both clinical and research contexts. Although translated into many languages, no validated Russian-language version has been available. Furthermore, the limited number of stimuli in the original RMET constrains its applicability in neuroimaging studies. This study presents ...
Added: September 13, 2026
Ребрейкина А. Б., Liaukovich K., Современная зарубежная психология 2024 Т. 13 № 2 С. 33–43
Создание методик диагностики на основе анализа движений глаз является особенно актуальным для оценки когнитивных функций у детей, которые не могут дать речевой или моторный ответ в силу своего возраста или из-за нарушений развития. Для таких детей использование объективных методов оценки когнитивных функций на основе айтрекинга может стать полезным диагностическим инструментом, дополняющим традиционные поведенческие опросники и ...
Added: September 8, 2026
Ребрейкина А. Б., Liaukovich K., Современная зарубежная психология 2023 Т. 12 № 4 С. 51–61
Активное развитие метода видеоокулографии, т. е. регистрации движений глаз, создает возможности для создания новых подходов для развития когнитивных функций и обучения. Методики, основанные на видеоокулографии, могут быть полезны не только для детей с серьезными нарушениями моторных функций, но и детей с нарушениями развития когнитивных функций, прежде всего внимания, так как контроль движений глаз тесно связан ...
Added: September 8, 2026
Портнова Г. В., Liaukovich K., Васильева Л. Н. et al., Журнал высшей нервной деятельности им. И.П. Павлова 2022 Т. 72 № 4 С. 504–519
Когнитивная и эмоциональная нагрузка в ходе увеличения сложности задач приводит к активации различных звеньев вегетативной нервной системы и может сопровождаться как увеличением эффективности решения задач, так и способствовать дестабилизации эмоционального состояния и уменьшению продуктивности. Увеличение когнитивной нагрузки при условии высокой мотивации испытуемых является стрессовым фактором и выражается в различной реакции симпатического и парасимпатического звеньев в ...
Added: September 8, 2026
Shaitan A., Ke Y., Hongjun Y. et al., PLoS Computational Biology 2026 Article e1013836
The interaction between peptides and human leukocyte antigen class II (HLA-II)
molecules plays a pivotal role in adaptive immune responses, as HLA-II mediates
the recognition of exogenous antigens and initiates T cell activation through peptide
presentation. Accurate prediction of peptide-HLA-II binding serves as a cornerstone
for deciphering cellular immune responses, and is essential for guiding the optimization
of antibody therapeutics. ...
Added: September 7, 2026
Proskurnina E., Liaukovich K., Bychkovskaya L. et al., Metabolites 2023 Vol. 13 No. 1 P. 73
Added: September 7, 2026
Liaukovich K., Sazhin S., Bobrov P. et al., International Journal of Molecular Sciences 2022 Vol. 23 No. 19 P. 11785
Added: September 7, 2026
Liaukovich K., Ukraintseva Y., Martynova O., Neuropsychologia 2022 Vol. 165 P. 108129
Added: September 7, 2026
Alshanskaia E., Portnova G., Liaukovich K. et al., Frontiers in Neuroscience 2024 Vol. 18
Added: September 7, 2026
Oknina L. B., Podlepich V. V., Sieber I. et al., Human Physiology 2026 Vol. 52 No. 3 P. 295–301
The aim of this study was to evaluate the potential use of the frequency-following response (FFR) for assessing preserved perception of simple speech stimuli in humans. To this end, event-related potentials (ERPs) elicited by isolated Russian syllables were recorded and analyzed in 29 healthy subjects in two frequency ranges, the standard range (1—40 Hz) and ...
Added: September 6, 2026
Момджян К. Х., Кржевов В. С., Poddiakov A. et al., М.: Новые печатные технологии, 2025.
При всей кажущейся ясности и недвусмысленности понятия «свобода» дать ему исчерпывающую научную дефиницию весьма затруднительно. У каждой отрасли знания свой подход к трактовке этого многоаспектного понятия. В ходе обсуждения, материалы которого вошли в настоящий сборник, свобода рассматривается с позиций философии, социологии, юриспруденции, психологии, нейрофизиологии и т.д.
Участники дискуссии рассуждают о границах свободы воли и субъектности человека, о соотношении свободы и ...
Added: September 6, 2026
Liaukovich K., Panfilova E., Khayrullina G. et al., International Journal of Psychophysiology 2025 Vol. 207 P. 112475
Added: September 5, 2026
Portnova G., Liaukovich K., Sharaev M., Cognitive Neurodynamics 2025 Vol. 19 Article 181
Added: September 5, 2026
Liaukovich K., Bainbridge E., Khayrullina G. et al., Neuroscience and Behavioral Physiology 2026 Vol. 56 P. 10–19
Added: September 5, 2026
Knyazev E., Timur Kulagin, Antipenko I. et al., Placenta 2026 Vol. 176 P. 1–12
Background. Preeclampsia (PE) complicates 2–8% of pregnancies and involves placental hypoxia and HIF-pathway activation, especially in early-onset PE (eoPE). Chemical mimetics like cobalt(II) chloride (CoCl2) and oxyquinoline derivatives model trophoblast hypoxia in vitro, yet their fidelity in recapitulating PE gene profiles remains unclear. Integrating patient tissue analyses with experimental models may reveal common markers and ...
Added: December 22, 2025
Mironova P., Меркулова А. А., Фольклор и антропология города, Российская Федерация 2025 Т. 7 № 3 С. 74–95
Traditionally, Delhi’s markets are oversaturated spaces for sensory perception: a cacophony of sounds, contrasting smells, and a vivid visual cascade. This study adopts a sensitive ethnographic lens to examine how bodily sensations relate to sociality, of- fering insight into the social order of the marketplace and an individual’s position within it. What markers evoke comfort ...
Added: November 9, 2025
Mikhail V. Meshcheryakov, Nina I. Zhukova, Regular and Chaotic Dynamics 2025 Vol. 30 No. 1 P. 141–154
Continuous actions of topological semigroups on products $X$ of an arbitrary family of topological spaces $X_i$, $i\in J,$ are studied. The relationship between the dynamical properties of semigroups acting on the factors $X_i$ and the same properties of the product of semigroups on the product of these spaces is investigated. We consider the following dynamical properties: ...
Added: February 18, 2025
Velikonivtsev F., Головкин А. С., Российский кардиологический журнал 2020 Т. 25 № 10 С. 221–231
Extracellular vesicles are biological membrane-coated objects with size less then 1000 nm. They can contain variety of biologically active molecules (such as proteins, miRNA, mRNA, DNA etc.) and also are able to provide intercellular communications and implement lots if biological functions. Now possibilities of using extracellular vesicles for therapeutic approaches against various diseases and pathological ...
Added: March 5, 2024
Zhukova N., Regular and Chaotic Dynamics 2024 Vol. 29 No. 1 P. 174–189
The focus of the work is the investigation of chaos and closely related dynamic properties of continuous actions of almost open semigroups and $C$-semigroups. The class of dynamical systems $(S, X)$ defined such semigroups $S$ is denoted by $\frak A.$ These semigroups contain, in particular, cascades, semiflows and groups of homeomorphisms. We extend the Devaney ...
Added: February 11, 2024
Makarova J., Maltseva D., Tonevitsky A., Frontiers in Molecular Biosciences 2023 Vol. 10 Article 1327985
Since its original discovery over a decade ago, extracellular RNA (exRNA) has been found in all biological fluids. Furthermore, extracellular microRNA has been shown to be involved in communication between various cell types. Importantly, the exRNA is protected from RNases degradation by certain carriers including membrane vesicles and non-vesicular protein nanoparticles. Each type of carrier ...
Added: November 29, 2023