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First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors
International Journal of Molecular Sciences. 2024. Vol. 25. No. 13. Article 7121.
Abstract
Aspergillus fungi constitute a pivotal element within ecosystems, serving as both contributors of biologically active compounds and harboring the potential to cause various diseases across living organisms. The organism’s proteolytic enzyme complex, termed the degradome, acts as an intermediary in its dynamic interaction with the surrounding environment. Using techniques such as genome and transcriptome sequencing, alongside protein prediction methodologies, we identified putative extracellular peptidases within Aspergillus ochraceus VKM-F4104D. Following manual annotation procedures, a total of 11 aspartic, 2 cysteine, 2 glutamic, 21 serine, 1 threonine, and 21 metallopeptidases were attributed to the extracellular degradome of A. ochraceus VKM-F4104D. Among them are enzymes with promising applications in biotechnology, potential targets and agents for antifungal therapy, and microbial antagonism factors. Thus, additional functionalities of the extracellular degradome, extending beyond mere protein substrate digestion for nutritional purposes, were demonstrated.
Derkacheva A., Sakirkina M., Kraev G. et al., /. 2026.
Comprehensive data on natural hazards and their consequences are crucial for effective for risk assessment, adaptation planning, and emergency response. However, many countries face challenges with fragmented, inconsistent, and inaccessible data, particularly regarding local-scale events. To address this data gap in Russia, we developed an end-to-end processing pipeline that scrapes news from various online sources, ...
Added: April 28, 2026
Кивалов С. Н., Fitzjarrald D., Boundary-Layer Meteorology 2018 Vol. 166 No. 2 P. 165–198
Cloud shadows lead to alternating light and dark periods at the surface, with
the most abrupt changes occurring in the presence of low-level forced cumulus clouds.
We examine multiyear irradiance time series observed at a research tower in a midlatitude
mixed deciduous forest (Harvard Forest, Massachusetts, USA: 42.53◦N, 72.17◦W) and one
made at a similar tower in a tropical ...
Added: April 24, 2026
Кивалов С. Н., Fitzjarrald D., Boundary-Layer Meteorology 2019 Vol. 173 No. 1 P. 1–52
We examine the physical and biological responses of forest canopies to step changes in light
caused by passing low cumulus clouds that intermittently block the direct solar beam. Using
data obtained at a tropical rainforest and at a midlatitude deciduous forest, we estimate the
course of sensible heat flux, net ecosystem exchange, evapotranspiration, and water-use efficiency in response ...
Added: April 24, 2026
Addressing effects of environment on eddy-covariance flux estimates at a temperate sedge-grass marsh
Кивалов С. Н., Dušek J., Czerný R. et al., Boundary-Layer Meteorology 2023 Vol. 186 P. 217–250
We examined the net-ecosystem-exchange (NEE)-based annual carbon-balance estimates
obtained from eddy-covariance (EC) measurements at an unmanaged sedge-grass marsh
ecosystem (Tˇreboˇn, Czech Republic, 49°1′ N, 14°46′ E), seeking methods to improve the EC
measurements in inhomogeneous environment. The data filtering procedure was developed
using three thresholds: (a) a stationarity test; (b) a stability u∗-threshold; and (c) a high relative
humidity RH-threshold. ...
Added: April 24, 2026
Кивалов С. Н., Лопес де Гереню В. О., Хорошаев Д. А. et al., Forests 2023 Vol. 14 No. 8 Article 1568
Soil respiration (SR) is a main component of the carbon cycle in terrestrial ecosystems,
and being strongly affected by changes in the environment, it is a good indicator of the ecosystem’s
ability to cope with climate change. This research aims to find better empirical SR models using
25-year-long SR monitoring in two forest ecosystems formed on sandy Entic ...
Added: April 24, 2026
Касицкий В. А., Кивалов С. Н., Лопес де Гереню В. О. et al., АгроЭкоИнфо 2025 Т. 5 Статья 517
Оценка дыхания почвы - одного из основных источников углекислого газа в атмосферу – необходима для корректного определения углеродного баланса в наземных экосистемах. Сравнение пульсационного и камерного методов измерения дыхания почвы, проведенные в березняке постагрогенном в регионе южного Подмосковья, показывает хорошую сопоставимость этих методов с коэффициентом детерминации R2=0.75 (p-value=7.546e-12). С учетом высокой пространственной и временной неоднородности ...
Added: April 24, 2026
Кивалов С. Н., Ecological Modelling 2024 Vol. 498 Article 110904
On certain kinds of cloudy days, many forested ecosystems exhibit enhanced carbon uptake and water-use efficiency-the cloudy-day forest flux anomaly. Using ensemble methods to analyze eddy-covariance fluxes, we have
diagnosed net ecosystem exchange (NEE) and water-use efficiency (WUE) of a temperate broadleaf forest and a
tropical evergreen forest as they responded to natural fluctuating-light regimes. Here we ...
Added: April 24, 2026
Кивалов С. Н., Курганова И. Н., Быховец С. С. et al., Soil Systems 2025 Vol. 9 No. 1 Article 9010025
Carbon dioxide (CO₂) efflux from soil (or soil respiration, SR) is one of the most important yet variable characteristics of soil. When evaluating large areas, CO₂ efflux modeling serves as a viable alternative to direct measurements. This research aims to identify site-specific differences and their effects on empirical CO₂ efflux modeling. The experimental data from ...
Added: April 24, 2026
G.V.Klink, Semenkov I. N., Enchilik P. R., Science of the Total Environment 2025 Vol. 998 Article 180219
Natural regeneration of forest soils affected by agricultural practices is a common phenomenon in many regions worldwide, particularly in boreal ecosystems. Affecting soil quality, the partitioning of extractable fractions of heavy metals and metalloids (HMMs) is not studied during natural regeneration. The following stages of natural regeneration after plowing have been identified in the Smolenskoye ...
Added: April 19, 2026
Гаранкина Е. В., Шоркунов И. Г., Лобков В. А. et al., Геоморфология и палеогеография (ранее - Геоморфология) 2025 Т. 56 № 4 С. 678–700
Late Pleistocene transformation of European glacial landscapes is often narrowed down to the impact of
postglacial linear erosion. Yet in the marginal zone of MIS 6 glaciation extensive watersheds were protractedly
affected by the postglacial flattening in periglacial and interglacial conditions. Local sediment sinks such
as kettle holes and dry valleys infilled throughout the postglacial stage can serve ...
Added: April 18, 2026
Куракова А. А., Е.Д. Павлюкевич, Большаков Д. В., Вестник Московского университета. Серия 5: География 2025 Т. 80 № 4 С. 40–48
В последние десятилетия изменение климата и деятельность человека оказывают все большее воздействие на реки, что во многом может провоцировать или усиливать проявление опасных русловых процессов (размывов берегов). Доступность спутниковых снимков с глобальным охватом предоставляет широкие возможности для изучения плановых деформаций русла и их количественной оценки. Это все стало основой для активного развития методов дистанционного зондирования, ...
Added: April 3, 2026
Pavlyukevich Ekaterina D., Krylenko I., Motovilov Y. et al., Water Resources 2025 Vol. 52 No. 2 P. 159–171
A set of hydrometeorological models, has been used to assess the likely changes in Terek River runoff in the 21st century, taking into account climate and glaciation changes in the basin. It has been shown that runoff changes will range from –2 to +5% under the RCP 2.6 scenario and from –8 to +14% under ...
Added: April 3, 2026
S. S. Bricheva, P. M. Shilov, Yurchenko A. P. et al., Eurasian Soil Science 2025 Vol. 58 Article 74
The high variability of properties in urban soils and the abundance of anthropogenic inclusions that interfere with the propagation of electromagnetic fields are the reasons why they are seldom studied by geophysics. At the same time, geophysics is an efficient and fast way to diagnose soil structure and dynamics without affecting the functioning of the ...
Added: March 26, 2026
Sheremetskaya E. D., Karevskaya I. A., Samus’ A. V. et al., Moscow University Geology Bulletin 2024 Vol. 79 No. S1 P. S100–S109
For decades the northeastern slope of the Borisoglebsk Upland has been a focal point for natural scientists interested in geochronology and Quaternary environmental reconstructions. A key reference section of the Upper Volga River region is a set of geological cores, exposures and excavations on the sides of the Puzhbol Gully system. The ORV section (Eastern ...
Added: March 21, 2026
Лобков В. А., Шоркунов И. Г., Гаранкина Е. В. et al., Почвоведение 2025 № 1 С. 102–119
Magnetic properties of soils depend on the initial properties of soil-forming material and a number of post-sedimentary processes. Within the uplands in the center and south of the East European Plain, the heterogeneity of spatial distribution of magnetic properties correlates closely with the soil-cover heterogeneity caused by the paleocryogenic microtopography. Differences in magnetic susceptibility are ...
Added: March 21, 2026
E.V. Garankina, Посаженникова В. С., Шоркунов И. Г., News of Russian Science Academy. Geographic serie 2025 Vol. 89 No. 4 P. 578–603
Landscapes of Сentral European Russia were sculpted by the last Middle Pleistocene glaciation that constrained most of the following sedimentation in successive interglacial and periglacial settings to various depressions of the glacial paleolandscape. However, corresponding continuous postglacial sequences are extremely rare in the region due to the dramatic fluctuations of the regional base level and ...
Added: March 21, 2026
Zatsepin A. G., Podymov O. I., Myslenkova K.P. et al., Oceanology 2025 Vol. 65 P. 803–815
Abstract
The article studies patterns and dynamics of the upper mixed layer (UML), its temperature and thickness during the warm period (April–November). The initial CTD data were collected during regular measurements in the active layer (AL) at the Gelendzhik research site, in the shelf–slope area of the northeastern Black Sea, in 2010–2023. The heat, buoyancy, and ...
Added: March 12, 2026
Wentai L., Song H., Li Q. et al., Palaeogeography, Palaeoclimatology, Palaeoecology 2025 Vol. 666 Article 112836
Since the late 20th century, global warming has resulted in the significant retreat of alpine glaciers in high-altitude mountainous regions worldwide. However, the glaciers in the Karakoram Mountains of Central Asia have remained stable or even expanded over recent decades, a phenomenon termed the “Karakoram Anomaly”. Here, we use tree-ring width of Picea schrenkiana growing at high altitudes of the Karakoram Mountains, to ...
Added: March 10, 2026
Shestakova A., Surkova D., Fatkulin A. et al., Microbiology Resource Announcements 2024 Vol. 13 No. 11 Article 13:e00416-24
Aspergillus ochraceus VKM-F4104D (L-1) is a saprotrophic fungus isolated
from buried soils of Phanagoria (Russia). This strain is known as a producer of the
fibrinolytic peptidase-activating plasma protein C. We have sequenced and assembled
its genome for a more detailed understanding of the fungus’ physiology and encoded
peptidases. ...
Added: January 20, 2026
S. N. Timorshina, E. A. Ganetskaya, A. A. Shestakova et al., Microbiology 2025 Vol. 94 No. 2 P. 199 – 209
The ability of the micromycete Tolypocladium inflatum to synthesize keratinolytic enzymes has been demonstrated for the first time. Twenty two strains of micromycetes isolated from enrichment cultures of keratinolytic microorganisms belonged to the genera Aspergillus, Cladosporium, Fusarium, Keratinophyton, Penicillium, Pseudallscheria, Tolypocladium, and Trichoderma. Only two strains showed high keratinolytic activity under submerged fermentation conditions: Keratinophyton terreum C106 (74.2 U) and Tolypocladium inflatum ST1 (87.1 U). The target activity of K. ...
Added: January 14, 2026
E.A. Popova, A.A. Shestakova, D.E. Surkova et al., Applied Biochemistry and Microbiology 2025 Vol. 61 No. 7 P. 1287–1295
The enzymes that are least represented but most in demand in biotechnology are highly specific proteases that can hydrolyze difficult-to-degrade substrates. Micromycetes from the genus Aspergillus have been identified as producers of these proteases, which have diverse biochemical properties. For Aspergillus ochraceus VKM F-4104D, which has been previously studied as a producer of a protease that activates protein C ...
Added: January 14, 2026