Экспрессия иммуно-ассоциированных генов в изолированных классических моноцитах при преэклампсии

Вестник Российского университета дружбы народов. Серия: Медицина. 2025. Т. 29. № 3. С. 335–352.

Вишнякова П. А., Киселева В. В., Полтавец А. С., Карягина В. Е., Багдасарян А. А., Knyazev E., Муминова К. Т., Ходжаева З. С., Ельчанинов А. В., Сухих Г. Т.

Relevance. Preeclampsia is a severe complication of pregnancy, occurring in 8% of cases and causing high maternal and perinatal mortality and morbidity. The etiology of preeclampsia is still a subject of research, however, more and more data indicate the involvement of innate immune cells, monocytes, in the pathogenesis of this dangerous pathology. The aim of our work was to study the expression of a number of immune-associated genes in classical blood monocytes in preeclampsia and physiological pregnancy. Materials and methods. The work used the methods of gradient centrifugation, magnetic sorting, cytometric analysis, real-time PCR. Results. We obtained a polar change in the interleukin-receptor axis for CXCL8 and its receptor CXCR1. In the first case, we observed a decrease in its relative level in PE, while the expression of its receptor increased. We also found a decrease in the expression of the pseudogene ADGRE4P in PE. Discussion and conclusions. The role of individual monocytic subpopulations in the development of PE is still being clarified. It is obvious that monocytes can change the cytokine profile of blood plasma of patients with PE, enhance the reactions of innate immunity, including inflammation. The CXCL8-CXCR1 axis may become a potential therapeutic target in the treatment of this dangerous gestational pathology.

Keywords: Preeclampsia, Преэклампсия, цитометрия, real-time PCR, monocytes, CD14, cytometry, моноциты, CD14, ПЦР в реальном времени