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A Novel Approach for Assessing the Proteolytic Potential of Filamentous Fungi on the Example of Aspergillus spp.
Microbiology and Biotechnology Letters. 2023. Vol. 51. No. 4. P. 457– 464.
Proteolytic enzymes produced by filamentous fungi can degrade various fibrous and globular proteins along with other metabolites that may also find application in biotechnology. In this study, the effect of proteolytic enzymes of 22 Aspergillus strains on various proteins was investigated using protein-containing diagnostic media. Subsequently, a new parameter estimating secreted proteinases specificity towards fibrous or globular proteins without its advanced biochemical research - index of severity of proteolytic action (ISPA) - was suggested. This index determines mycozymes specificity in following manner: its value increases with greater affinity to fibrous proteins, decreases if there is higher affinity to globular proteins. ISPA value was the lowest (0.52) for Aspergillus domesticus, indicating the highest specificity to globular proteins, the highest one (1.26) for A. glaucus, whose proteinases best hydrolyzed fibrous proteins. However, the highest overall proteolytic potential was observed for Aspergillus melleus. The ability to produce acid, alkali and extracellular pigments was evaluated for all isolated strains as well.
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Четверикова М. М., Потемкин А. Н., Stepanova E., Бюллетень экспериментальной биологии и медицины 2026 Т. 182 № 9 С. 242–247
Gemcitabine, a first-line chemotherapeutic agent for bladder cancer (BC), often loses clinical efficacy due
to the development of drug resistance, the underlying mechanisms of which remain poorly understood. In
this work, a highly gemcitabine-resistant RT112/GR cell line was obtained by long-term selection. The MTT
assay revealed a 9312-fold decrease in gemcitabine sensitivity and moderate resistance to cisplatin (2.60-fold,
p ...
Added: October 26, 2026
Maltseva D., Tonevitsky A., Frontiers in Molecular Biosciences 2026 Vol. 13 P. 1–28
MicroRNA (miRNA) genes are frequently located in close proximity within the genome, forming so-called clusters. These clusters are transcribed as polycistronic units, suggesting coordinated transcriptional regulation of the miRNAs they contain. However, individual mature miRNAs from the same cluster often accumulate at varying levels and exhibit distinct tissue-specific expression patterns, indicating the presence of regulatory ...
Added: October 23, 2026
Vasilyev A., Svirin EP, Dubynin I. et al., Cortex 2025 Vol. 189 P. 76–96
Added: October 22, 2026
Karina V. Klycheva, Mariia O. Silkina, Dmitry M. Hushpulian et al., Biochemistry. Biokhimiia 2026 Vol. 91 P. 1842–1858
Involvement of the transcription factor Bach1 in various scenarios of cellular regulation is reviewed herein. To date, the most extensively studied role of Bach1 is that of a repressor of the antioxidant genetic program initiated by the Nrf2 factor. This program counteracts oxidative stress, which triggers both ferroptosis and the intrinsic apoptotic pathway. Bach1 functions unrelated ...
Added: October 22, 2026
Svirin E., Бердышев Д. А., Шишкин С. Л., Вестник Российского государственного медицинского университета 2026 № 3 С. 42–50
Quasi-movements (QMs), which are movement attempts minimized to the point of movement extinction, evoke EEG patterns similar to those of real movement. QMs can model attempted movement in healthy subjects and are therefore of interest as an alternative to motor imagery (MI) in rehabilitation brain–computer interfaces (BCIs), in which the attempted movement of the paralyzed ...
Added: October 22, 2026
Sitdikova K., Tsoy A., Chaprov K. et al., Journal of Neuropathology and Experimental Neurology 2026 Article nlag078
Understanding the earliest pathological changes in Alzheimer disease (AD) is critical for improving early intervention strategies. However, the relationship between amyloid plaque characteristics and early behavioral and molecular alterations remains unclear. We used 6-month-old female APPswe/PS1dE9 mice, a model of early amyloid-dominant pathology, to assess cognition and emotionality across a battery of behavioral tests. Amyloid ...
Added: October 22, 2026
Effects of Wheat Malt Extract on Molecular and Behavioral Markers in Aged APP/PS1 and Wild-Type Mice
Kassenova A., Svirin E., Sitdikova K. et al., International Journal of Molecular Sciences 2026 Vol. 27 No. 11 Article 4994
Added: October 22, 2026
Cespuglio R., Schmitt-Böhrer A., Deykin A. et al., Metabolites 2026 Vol. 16 No. 2 Article 117
Added: October 22, 2026
Logvenkov S. A., Известия РАН. Механика жидкости и газа 2026 № 3 С. 59–75
The problem of the evolution of cell volume fraction in a spherical aggregate composed of cells,
extracellular matrix, and fluid is considered. The aggregate is surrounded by extracellular matrix. The
problem formulation employs a multiphase model of a biological continuum, previously developed by the
author, consisting of two actively interacting solid phases and a fluid. The propagation of ...
Added: October 20, 2026
Liaukovich K., Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I.P. Pavlova 2026 Vol. 76 No. 5 P. 587–598
Added: October 19, 2026
Nersisyan A., Frontiers in Molecular Biosciences 2026 Vol. 13 Article 1995001
Antiviral activity is commonly summarized using endpoint measures such as EC50 or EC90, yet these quantities emerge from a dynamic biological system and depend on viral replication kinetics, host-cell properties, experimental design, and the assay used to quantify infection. Infectious-virus titers, viral RNA, infected-cell fractions, viability measurements, and immune readouts capture different components of the ...
Added: October 15, 2026
Тукачев Н. В., González-Jiménez M., Ramakrishnan G. et al., Physical Chemistry Chemical Physics 2021 Vol. 23 No. 23 P. 13250–13260
Low-frequency vibrations play an essential role in biomolecular processes involving DNA such as gene expression, charge transfer, drug intercalation, and DNA–protein recognition. However, understanding the vibrational basis of these mechanisms relies on theoretical models due to the lack of experimental evidence. Here we present the low-frequency vibrational spectra of G-quadruplexes (structures formed by four strands ...
Added: October 14, 2026
Батова О. Н., Савинецкая Л. Е., Проявка С. В. et al., Nature Conservation Research. Заповедная наука 2026 Т. 11 № 4 С. 25–39
Spermophilus suslicus is a threatened species listed in Red Data Books at all levels (from regional Red Data
Books to the IUCN Red List of Threatened Species), and it currently requires special conservation activity.
Over the past 30–50 years, its range has changed from continuous to mosaic, and its population has declined
everywhere. In 2015, in Russia experimental ...
Added: October 13, 2026
Temereva E., Zoologischer Anzeiger 2026 Vol. 325 P. 182–195
Brachiopoda is a group of animals, that is still insufficiently studied at the ultrastructural level, despite the significance of ultrastructure of tissues and organs for analysing the functional morphology, reconstructing the organisation and biology of numerous extinct brachiopods, and establishing relationships among major taxa. In the present study, we provide a revised reconstruction of the ...
Added: October 11, 2026
Shestakova A., Surkova D., Fatkulin A. et al., Microbiology Resource Announcements 2024 Vol. 13 No. 11 Article 13:e00416-24
Aspergillus ochraceus VKM-F4104D (L-1) is a saprotrophic fungus isolated
from buried soils of Phanagoria (Russia). This strain is known as a producer of the
fibrinolytic peptidase-activating plasma protein C. We have sequenced and assembled
its genome for a more detailed understanding of the fungus’ physiology and encoded
peptidases. ...
Added: January 20, 2026
S. N. Timorshina, E. A. Ganetskaya, A. A. Shestakova et al., Microbiology 2025 Vol. 94 No. 2 P. 199 – 209
The ability of the micromycete Tolypocladium inflatum to synthesize keratinolytic enzymes has been demonstrated for the first time. Twenty two strains of micromycetes isolated from enrichment cultures of keratinolytic microorganisms belonged to the genera Aspergillus, Cladosporium, Fusarium, Keratinophyton, Penicillium, Pseudallscheria, Tolypocladium, and Trichoderma. Only two strains showed high keratinolytic activity under submerged fermentation conditions: Keratinophyton terreum C106 (74.2 U) and Tolypocladium inflatum ST1 (87.1 U). The target activity of K. ...
Added: January 14, 2026
A. A. Osmolovskiy, A. A. Shestakova, D. E. Surkova et al., Moscow University Biological Sciences Bulletin 2023 Vol. 78 No. 2 P. 106 – 110
The spectrum of activity of extracellular proteases against proteins of a human hemeostasis system has been studied in five Aspergillus species (A. athecius, A. caespitosus, A. glaucus, A. tamarii, and A. wentii). No activity types similar to that of the components of a hemeostasis system (thrombin, plasmin, factor Xa, urokinase, and protein C), as well as any activator-type activity towards precursors ...
Added: May 20, 2024
Surkova D., Lavrenova V., Klyagin S. et al., Current Medical Mycology 2023 Vol. 9 No. 1 P. 8–13
In this study, the abilities of 15 novel Aspergillus species to produce proteases were screened, including activity against components of human hemostasis. The assessed activities included direct thrombin-, plasmin-, factor Xa-, urokinase-, and protein C-like-activities, as well as activating activities towards the aforementioned proteins with human blood plasma. This was determined using chromogenic peptide substrates ...
Added: May 20, 2024
Osmolovsky A., Schmidt L., Orekhova A. et al., Life 2021 Vol. 11 Article 782
In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active ...
Added: October 4, 2021
Osmolovskiy A., Orekhova A. V., Kreyer V. G. et al., Biochemistry, Supplemental Series B 2018 Vol. 12 P. 164–166
The protein C activator activity, determined in normal plasma by using A. ochraceus protease is comparable with the activity of a commercial protease analogue from the South American copperhead venom (Protac®). As in the case of Protac®, the A. ochraceus protease can be used for protein C determination in plasma with its reduced content. Comparison of the activator protein ...
Added: October 30, 2020