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Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: molecular heterogeneity and erythrophagocytosis
Biomedical Optics Express. 2019. Vol. 10. No. 8. P. 4220–4236.
Будылин Г. С., Ширшин Е. А., Якимов Б. П., Гоголева М., Семенов А., Родионов С., Новоселова М., Гайер А., Ковалев А., Бернакевич А., Фадеев В. В., Армаганов А., Драчев В., Горин Д., Дарвин М., Щеславский В., Приезжев А. В.
Blood cell analysis is one of the standard clinical tests. Despite the widespread use of exogenous markers for blood cell quantification, label-free optical methods are still of high demand due to their possibility for in vivo application and signal specific to the biochemical state of the cell provided by native fluorophores. Here we report the results of blood cell characterization using label-free fluorescence imaging techniques and flow-cytometry. Autofluorescence parameters of different cell types – white blood cells, red blood cells, erythrophagocytic cells – are assessed and analyzed in terms of molecular heterogeneity and possibilities of differentiation between different cell types in vitro and in vivo.