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Regular version of the site

Article

Effect of Gold Nanoparticles on Mouse Spermatogenesis

Biology Bulletin. 2012. Vol. 39. No. 3. P. 229-236.
Makarov A. A., Zakhidov S., Pavlyuchenkova S., Marshak T., Rudoy V., Evdokimov Y., Dement'eva O., Zelenina I., Skuridin S., Khoklov A.

The response of mouse male germ cells exposed to gold nanoparticles (~ 2.5 nm) was studied. Our investigation demostrates that treatment with Au nanoparticles for four days does not impair the architecture of the spermatogenic epithelium. Cytogenetic evaluation using micronucleus assay showed that gold nanoparticles can affect the chromosomes of early primary spermatocytes. However, gold nanoparnicles did not induce chromosome abnomalities in spermatogonial stem cells. Further, the cauda epididymal sperm was isolated on the 14th day after treatment and was incubated in SDS solution (Na sodium dodecyl) and then in a solution containing DTT (dithiothreitol) to induce nuclear chromatin decondensation. Observations showed that after four days  of treatment of spermiogenic (postmeiotic) cells with gold nanoparticles the decondensation process had no differences from the control.