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Найдено 10 публикаций
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Статья
Makarov A. A., Zelenina I., Zakhidov S. et al. Biology Bulletin. 2018. Vol. 45. No. 2. P. 119-125.
Добавлено: 23 августа 2018
Статья
Захидов С. Т., Павлюченкова С. М., Зеленина И. А. et al. Biology Bulletin. 2012. Vol. 39. No. 3. P. 229-236.

The response of the mouse male germ cells exposed to gold nanoparticles (~2.5 nm) was studied. Our investigation demonstrates that treatment with Au nanoparticles for four days does not impair the architecture of the spermatogenic epithelium. Cytogenetic evaluation using micronucleus assay showed that gold nanoparticles can affect the chromosomes of early primary spermatocytes. However, gold nanoparticles did not induce chromosome abnormalities in spermatogonial stem cells. Further, the cauda epididymal sperm was isolated on the 14th day after treatment and was incubated in SDS solution (Na dodecyl sulphate) and then in a solution containing DTT (dithiothreitol) to induce nuclear chromatin decondensation. Observations showed that after four days of treatment of spermiogenic (postmeiotic) cells with gold nanoparticles the decondensation process had no differences from the control. On the contrary, in the experiment with the same cells and period of fixation but with a single exposure to gold nanoparticles, the number of mature gametes with totally decondensed nuclei reached 100% as opposed to 44% in the controls.

Добавлено: 11 ноября 2012
Статья
Makarov A. A., Zakhidov S., Pavlyuchenkova S. et al. Biology Bulletin. 2012. Vol. 39. No. 3. P. 229-236.

The response of the mouse male germ cells exposed to gold nanoparticles (~2.5 nm) was studied. Our investigation demonstrates that treatment with Au nanoparticles for four days does not impair the architecture of the spermatogenic epithelium. Cytogenetic evaluation using micronucleus assay showed that gold nanoparticles can affect the chromosomes of early primary spermatocytes. However, gold nanoparticles did not induce chromosome abnormalities in spermatogonial stem cells. Further, the cauda epididymal sperm was isolated on the 14th day after treatment and was incubated in SDS solution (Na dodecyl sulphate) and then in a solution containing DTT (dithiothreitol) to induce nuclear chromatin decondensation. Observations showed that after four days of treatment of spermiogenic (postmeiotic) cells with gold nanoparticles the decondensation process had no differences from the control. On the contrary, in the experiment with the same cells and period of fixation but with a single exposure to gold nanoparticles, the number of mature gametes with totally decondensed nuclei reached 100% as opposed to 44% in the controls.

Добавлено: 4 февраля 2013
Статья
Макаров А., Pavlyuchenkova S., Zakhidov S. et al. Biology Bulletin. 2012. Vol. 39. No. 3. P. 229-236.
Добавлено: 19 марта 2013
Статья
Makarov A. A., Zakhidov S., Pavlyuchenkova S. et al. Biology Bulletin. 2012. Vol. 39. No. 3. P. 229-236.
Добавлено: 20 марта 2013
Статья
Makarov A. A., Захидов С. Т., Рудой В. М. et al. Biology Bulletin. 2017. Vol. 44. No. 3. P. 233-236.

С помощью метода учета мейотических микроядер изучено действие ультрамалых наночастиц зо- лота (НЧЗ), в том числе в сочетании с алкилирующим мутагеном дипином на генетические струк- туры мужских половых клеток у мышей. Впервые показано, что НЧЗ в зависимости от выбранных условий эксперимента способны проявлять мутагенную, антимутагенную и комутагенную актив- ности.

Добавлено: 19 января 2018
Статья
Obozova. T., Zorina Z. Biology Bulletin. 2012. Vol. 39. No. 7. P. 601-617.
Добавлено: 3 декабря 2013
Статья
Makarov A. A., Pavlyuchenkova S., Marshak T. et al. Biology Bulletin. 2012. Vol. 39. No. 6. P. 504-514.

It has been shown that a single intratesticular injection of the chemical mutagen dipin (experiment) or saline (control) into mice resulted in significant but reversible morphohistological damage of the spermatogenic epithelium. However, unlike the controls, in mutagenized testes these damages were more pronounced. Thus, the process of restoring a normal pattern of spermatogenesis was slower. In addition, on day 35 of fixation, mature gametes were almost completely absent in the cauda epididymis and a large number of sperm cells with abnormal head shape (58.5 versus 1.7% in the controls) appeared in the testes. Using spermatogonial and meiotic micronucleus assay, we found that dipin did not induce a rise in the number of gross chromosomal mutations in the spermatogonial stem cells (SSCs): on days 35, 56, and 100 postinjection, the incidence of aberrant spermatogonia and round spermatids was not significantly different from the saline control. The degree of gametic chromatin decondensation was evaluated after treatment of the cauda epididymal sperm with sodium dodecyl sulfate (SDS) and dithiothreitol (DTT). Judging by the results of the in vitro sperm chromatin decondensation on days 7, 14, 35, 56, and 100 after the injection of dipin or saline, the number of decondensed nuclei decreased sharply in the studied samples as compared with the sperm from intact animals where sperm cells with fully decondensed chromatin prevailed.

Добавлено: 5 февраля 2013
Статья
Makarov A. A., Pavlyuchenkova S., Zakhidov S. et al. Biology Bulletin. 2012. Vol. 39. No. 6. P. 504-514.
Добавлено: 19 марта 2013
Статья
Lebedev Y., Bezryadnov, D., Deacon, R. et al. Biology Bulletin. 2013. Vol. 40. No. 2. P. 179-186.

The Effect of a Caudal Hippocampus Lesion on Learning in a Morris Water Maze in Bank Voles (Clethrionomys glareolus).

Добавлено: 17 ноября 2013