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Expression of CD44 Isoforms in Tumor Samples and Cell Lines of Human Colorectal Cancer
Bulletin of Experimental Biology and Medicine. 2022. Vol. 173. No. 1. P. 155–159.
Detection of colorectal cancer biomarkers (CRC) remains an urgent task for the diagnosis and prediction of the disease course. A promising approach is the study of cancer stem cell markers. The cell surface glycoprotein CD44 is very important for CRC and its stem cells. Alternative splicing of 9 variable exons of CD44 mRNA leads to the formation of various isoforms of the protein with different roles in the progression of cancer. Studies of the functions of CD44 isoforms require adequate models considering the distribution of CD44 isoforms in real tumor samples. In the present study, the expression profile of CD44 isoforms in CRC was assessed based on the publicly available mRNA sequencing data of patient tumors from the TCGA-COAD database. It was shown that normal tissues predominantly expressed isoforms 3 and 4 at nearly equal levels, whereas tumors mainly expressed isoforms 2, 3, and 4; isoform 3 was expressed at the highest level. Further, the most relevant cell lines for studying the role of CD44 in CRC were identified based on the analysis of mRNA sequencing data of 55 CRC cell lines form CCLE database. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
S. V. Nikulin, Olkhovik D., A.V. Razumovskaya et al., Doklady Biochemistry and Biophysics 2026 Vol. 526 P. 59–64
A novel trend in anticancer therapy is based on the combination of cytotoxic and metabolic drugs. Bach1 is a transcription factor activating glycolysis and increasing proliferation and metastatic potential in cancer cells. The cytotoxic effect was evaluated for a combination of rotenone, an inhibitor of mitochondrial respiration, and two different inhibitors of Bach1 transcription factor ...
Added: April 13, 2026
Yanova M., Maltseva D., Tonevitsky A., Frontiers in Cell and Developmental Biology 2025 Vol. 13 Article 1720996
Colorectal cancer (CRC) remains one of the leading causes of cancer-related morbidity and mortality worldwide. Tumor sidedness, distinguishing left- (LCRC) and right-sided (RCRC) cancers, has emerged as a critical clinical determinant, influencing patient prognosis and therapeutic response. However, the cellular and molecular mechanisms underlying these differences remain poorly understood. Recent advances in single-cell RNA sequencing ...
Added: December 12, 2025
Raigorodskaya M., Novosad V., Tonevitskaya S. et al., Applied Biochemistry and Microbiology 2022 Vol. 58 No. 9 P. 992 – 996
The expression profile of CD44 isoforms in 55 colorectal cancer (CRC) cell lines has been evaluated based on mRNA sequencing data from the Cancer Cell Line Encyclopedia database. The distribution of CD44 mRNA isoforms differs significantly between CRC lines. In 13 studied lines, including Caco-2 and RKO, CD44 expression was not detected. In most other ...
Added: September 1, 2025
Alexandra Razumovskaya, Maria Silkina, Poloznikov A. et al., Frontiers in Molecular Biosciences 2025 Vol. 12 Article 1531175
Colorectal cancer is characterized by an extremely high mortality rate, mainly caused by the high metastatic potential of this type of cancer. To date, chemotherapy remains the backbone of the treatment of metastatic colorectal cancer. Three main chemotherapeutic drugs used for the treatment of metastatic colorectal cancer are 5-fluorouracil, oxaliplatin and irinotecan which is metabolized ...
Added: January 13, 2025
D. M. Olkhovik, M. O. Silkina, A. V. Razumovskaya et al., Doklady Biochemistry and Biophysics 2025 Vol. 520 P. 25–28
Ferroptosis is an iron-dependent form of programmed cell death (PCD) associated with lipid membrane peroxidation. It has gained attention in cancer research because some tumor cells that are resistant to other forms of PCD are sensitive to ferroptosis. Despite the significant amount of research on ferroptosis, the list of known inducers remains limited, creating opportunities ...
Added: October 31, 2024
Razumovskaya A., Silkina M., Кулагин Т. А. et al., Биотехнология 2023 Т. 39 № 6 С. 73–83
To date, colorectal cancer (CRC) remains one of the most common malignant neoplasms worldwide with a high mortality rates. In recent decades, progress in the study of the mechanisms underlying drug resistance in CRC confirmed the need to search for new therapeutic approaches. In particular, tumor cells that are resistant to standard therapy and to ...
Added: February 13, 2024
Razumovskaya A., Silkina M., Nikulin S. et al., Клеточные технологии в биологии и медицине 2023 № 4 С. 283–288
The activity of known modulators of the Nrf2 signaling pathway (bardoxolone and brusatol) was studied on cultures of tumor organoids of metastatic colorectal cancer previously obtained from three patients. The effect of modulators has been studied both as monotherapy and in combination with standard chemotherapy drugs used to treat colorectal cancer. The Nrf2 inhibitor brusatol ...
Added: January 23, 2024
Maltseva D., Tonevitsky A., Frontiers in Molecular Biosciences 2023 Vol. 10 Article 1326148
Alternative splicing is often deregulated in cancer, and cancer-specific isoform switches are part of the oncogenic transformation of cells. Accumulating evidence indicates that isoforms of the multifunctional cell-surface glycoprotein CD44 play different roles in cancer cells as compared to normal cells. In particular, the shift of CD44 isoforms is required for epithelial to mesenchymal transition ...
Added: November 29, 2023
Everest-Dass A., Nersisyan S., Maar H. et al., Molecular Oncology 2024 Vol. 18 No. 1 P. 62–90
Hematogenous metastasis limits the survival of colorectal cancer (CRC) patients. Here, we illuminated the roles of CD44 isoforms in this process. Isoforms 3 and 4 were predominantly expressed in CRC patients. CD44 isoform 4 indicated poor outcome and correlated with epithelial-mesenchymal transition (EMT) and decreased oxidative phosphorylation in patients; opposite associations were found for isoform 3. Pan-CD44 ...
Added: November 1, 2023
Brovkina O., Никитин А. Г., Клиническая практика 2021 Т. 12 № 1 С. 66–71
Determination of the mutations' status in the KRAS and NRAS genes is a necessary requirement in the treatment of patients with colorectal cancer (CRC). Patients with certain mutations in the KRAS and NRAS genes are resistant to anti-EGFR drug therapy and have a lower median survival rate than those with WT (wild type) genotypes, that ...
Added: June 20, 2022
Novosad V., Maltseva D., Тоневицкий Е. А. et al., Клеточные технологии в биологии и медицине 2022 С. 49–54
Выявление биомаркеров колоректального рака (КРР) остаётся актуальной задачей для диа- гностики и прогнозирования течения заболевания. Перспективным подходом считается изу- чение маркеров раковых стволовых клеток. Гликопротеин клеточной поверхности CD44 имеет особое значение для колоректального рака и его раковых стволовых клеток. Альтернативный сплайсинг 9 вариабельных экзонов мРНК CD44 приводит к образованию различных изоформ белка, роли которых в ...
Added: March 14, 2022
Poloznikov A., Nikulin S., Bolotina L. et al., Frontiers in Pharmacology 2021 Vol. 12 Article 777114
Colorectal cancer (CRC) is one of the most common and lethal types of cancer. Although researchers have made significant efforts to study the mechanisms underlying CRC drug resistance, our knowledge of this disease is still limited, and novel therapies are in high demand. It is urgent to find new targeted therapy considering limited chemotherapy options. ...
Added: January 17, 2022
Nersisyan S., Galatenko A. V., Ahlers A. et al., Biochimie 2022 Vol. 192 P. 91–101
In this study we analyzed expression of CD24 in a cohort of colorectal cancer patients using immunohistochemistry staining of CD24. We found a significant association between absence or low expression of CD24 (10% of membranous and 55% of cytoplasmic staining) and shortened patient survival. Protein localization played a crucial role in the prognosis: membranous form ...
Added: October 23, 2021
Mal'tseva D.V., Makarova Yu. A., Raigorodskaya M. P. et al., Bulletin of Experimental Biology and Medicine, Switzerland 2019 Vol. 166 No. 3 P. 377–382
The effects of laminins 332 and 411 (LM-332 and LM-411) on the epithelial—mesenchymal transformation of colorectal cancer cells (lines HT-29, HCT-116, and RKO) with different metastatic potential were studied. Culturing of RKO cells on both laminins was associated with modification of the cell shape, which became more spindle-like or stellate, and with higher expression of ...
Added: October 31, 2020
Shkurnikov M. Yu., Nersisyan S. A., Osepyan A. S. et al., Doklady Biochemistry and Biophysics 2020 Vol. 493 No. 1 P. 208–210
Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of ...
Added: October 13, 2020
Maltseva D., Raygorodskaya М., Knyazev E. et al., Biochimie 2020 Vol. 174 P. 107–116
The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we ...
Added: June 8, 2020
Tonevitsky A., Maltseva D., BMC Medical Genomics 2018 Vol. 11 No. 1 P. 9
Background: Laminins are a major family of extracellular matrix proteins and the main component of basement membranes. Laminins are involved in many if not all stages of cancer progression, and expression of laminin genes has prognostic value in various types of cancer, including colorectal. Only single laminin genes or components of a single laminin trimer ...
Added: April 14, 2020