Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes

Osmolovskiy A.; Lukianova A.; Zvonareva E.; Kreyer V.; Baranova N.; Egorov N.

doi:10.1016/j.btre.2018.e00265

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Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes

Biotechnology Reports. 2018. Vol. 19. P. e00265.

Osmolovskiy A., Lukianova A., Zvonareva E., Kreyer V., Baranova N., Egorov N.

A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.

Keywords: micromycetes

Количественная оценка развития колонии микромицетов на поверхностях полимеров и композитов на их основе

Исаев Е. А., Доронин Ф. А., Евдокимов А. Г. et al., Математическая биология и биоинформатика 2021 Т. 16 № 2 С. 367–379

The need to ensure the possibility of widespread use of electronic and mobile health-saving technologies requires not only the formation of an appropriate information technology infrastructure and the development of effective algorithms for processing a large amount of personal information, but also involves the creation of innovative biologically compatible materials that allow sensors and medical ...

Added: October 28, 2021

Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete Aspergillus ochraceus Capable of Activating Protein C of Blood Plasma

Komarevtsev Sergei K., Evseev P., Shneider M. et al., Microorganisms 2021 Vol. 9 No. 9 Article 1936

Micromycetes are known to secrete numerous enzymes of biotechnological and medical potential. Fibrinolytic protease-activator of protein C (PAPC) of blood plasma from micromycete Aspergillus ochraceus VKM-F4104D was obtained in recombinant form utilising the bacterial expression system. This enzyme, which belongs to the proteinase-K-like proteases, is similar to the proteases encoded in the genomes of Aspergillus fumigatus ATCC MYA-4609, A. oryzae ATCC ...

Added: October 4, 2021

Microbial features of newly-formed soils of disposal fields from sugar refineries

Mikheev P., Zamotaev I., Telnova N., , in: E3S Web of Conferences Volume 265 (2021) Actual Problems of Ecology and Environmental Management (APEEM 2021)Vol. 265: Actual Problems of Ecology and Environmental Management (APEEM 2021).: EDP Sciences, 2021. Ch. 03009 P. 1–5.

Newly-formed soils of wastewater disposal fields from two sugar refineries in forest-steppe zone (Kursk region, European Russia) were studied. Among factors of soil formation on sugar wastewater lagoons the geochemical influence of wastewater mixed with diluted filtration and transport-washing sludge stands out. We have revealed key physico-chemical and microbiological properties of such soils formed under ...

Added: July 2, 2021