Роль белок-липидных взаимодействий в функционировании битопных мембранных белков

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Роль белок-липидных взаимодействий в функционировании битопных мембранных белков

С. 45–46.

Бочаров Э. В., Лесовой Д. М., Минеев К. С., Бочарова О. В., Урбан А. С., Бершацкий А. В., Надеждин К. Д., Волынский П. Е., Efremov R., Арсеньев А. С.

Language: Russian

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Publication based on the results of:

Supercomputer technologies of atomic modeling in the material science and physics of biomembranes (2019)

In book

II объединенный научный форум: VI съезд физиологов СНГ, VI съезд биохимиков России, IX российский симпозиум «Белки и пептиды» (Сочи, Дагомыс, 1–6 октября 2019). Научные труды.

Т. 2. , М.: Издательство "Перо", 2019.

Diverse Interactions of Sterols with Amyloid Precursor Protein Transmembrane Domain Can Shift Distribution Between Alternative Amyloid-β Production Cascades in Manner Dependent on Local Lipid Environment

Volynsky P., Urban A., Pavlov K. et al., International Journal of Molecular Sciences 2025 Vol. 26 No. 2 Article 553

Alzheimer’s disease (AD) pathogenesis is correlated with the membrane content of various lipid species, including cholesterol, whose interactions with amyloid precursor protein (APP) have been extensively explored. Amyloid-β peptides triggering AD are products of APP cleavage by secretases, which differ depending on the APP and secretase location relative to ordered or disordered membrane microdomains. We used high-resolution NMR to probe ...

Added: October 12, 2025

Effect of low molecular weight chitosan on cells of epidermis from pea leaves

Kiselevsky D. B., Shagdarova B. T., Varlamov V. P. et al., Moscow University Biological Sciences Bulletin 2021 Vol. 76 No. 1 P. 14–19

The effect of low molecular weight chitosans with a molecular weight (MW) of 5, 6, 10, 25, and 45 kDa and a deacetylation degree (DD) of 85–99% obtained by chemical depolymerization and enzymatic hydrolysis on the cells of the epidermis of pea leaves was analyzed. Low molecular weight chitosans induced damage to guard cells' plasma membrane, ...

Added: October 14, 2022

Use of a laser interference microscope for estimating fluctuations and the equivalent elastic constant of cell membranes

Yusipovich A., Parshina E., Baizhumanov A. A. et al., Instruments and Experimental Techniques 2021 Vol. 94 No. 6 P. 877–885

An automated interference microscope has been used to determine the rms amplitude of fluctuations of living cells in vitro. The geometric thickness of cells has been calculated based on the measured optical path differences of light waves and the equivalent elastic constants of these cells have been estimated. The determined rms amplitude of fluctuations of the optical ...

Added: November 15, 2021

Использование лазерного интерференционного микроскопа для оценки флуктуаций и эквивалентной константы упругости мембран клеток

Юсипович А. И., Паршина Е. Ю., Иванов А. Д. et al., Приборы и техника эксперимента 2021 № 6 С. 93–101

Описано применение автоматизированного интерференционного микроскопа для определения среднеквадратичной амплитуды флуктуаций живых клеток in vitro. Измеренные оптические разности хода световых волн использованы для расчета геометрических толщин клеток. Оценены эквивалентные константы упругости таких клеток. Полученные значения среднеквадратичной амплитуды флуктуаций оптической разности хода составили 0.3–2.7 нм, что соответствует 4–40 нм среднеквадратичной амплитуды флуктуаций мембраны. Показано, что амплитуды флуктуаций ...

Added: November 2, 2021

Conformational variability and intramembrane recognition possibly associated with abnormal cleavage of APP transmembrane domain

Urban A., Bocharov E., Nadezhdin K. et al., , in: FEBS Open Bio Volume 9, Issue S1 Supplement: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019Vol. 9: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019.: Oxford: Wiley, 2019. Ch. 31-016 P. 299–299.

Bitopic proteins having only one helical transmembrane (TM) domain are a class of biologically significant membrane proteins, including the type I receptors and amyloid precursor protein (APP), which are involved in regulating the homeostasis of human organism and recognized as substrate by c-secretase. Amyloid Ab-peptides forming plaques in brain during Alzheimer disease (AD) are the ...

Added: November 1, 2019

Familial L723P Mutation Can Shift the Distribution between the Alternative APP Transmembrane Domain Cleavage Cascades by Local Unfolding of the Ε‑Cleavage Site Suggesting a Straightforward Mechanism of Alzheimer’s Disease Pathogenesis.

Bocharov E., Nadezhdin K., Urban A. et al., ACS Chemical Biology 2019 Vol. 14 No. 7 P. 1573–1582

Alzheimer’s disease is an age-related pathology associated with accumulation of amyloid-β peptides, products of enzymatic cleavage of amyloid-β precursor protein (APP) by secretases. Several familial mutations causing early onset of the disease have been identified in the APP transmembrane (TM) domain. The mutations influence production of amyloid-β, but the molecular mechanisms of this effect are ...

Added: August 8, 2019

Proteins and membranes: born to be together. A computational view

Polyansky A., Kuznetsov A., Volynsky P. et al., , in: Biomembranes 2018. International Conference. 1-5 October 2018. Book of Abstracts.: Dolgoprudni: MIPT, 2018. P. 89–90.

A computational approach to the analysis of structural and dynamical properties of all components of model membranes - membrane proteins, lipids, water and ions - has been developed. It is established that local changes in the membrane environment play an important role in the binding of membrane-active peptides and peripherical membrane proteins, causing specific clustering ...

Added: October 23, 2018

Probing the effect of membrane contents on transmembrane protein-protein interaction using solution NMR and computer simulations

Bragin P. E., Kuznetsov A.S., Bocharova O. V. et al., Biochimica et Biophysica Acta - Biomembranes 2018 Vol. 1860 No. 12 P. 2486–2498

The interaction between the elements of the secondary structure is the key process, determining the spatial structure and activity of a membrane protein. The transmembrane (TM) helix-helix interaction is known to be especially important for the function of so-called type I or bitopic membrane proteins, which have small TM domains, consisting of a single ⍺-helix. ...

Added: September 25, 2018

Молекулярное моделирование биомембран и их комплексов с трансмембранными α-спиралями белков

Smirnov K., Kuznetsov A., Антонов М. Ю. et al., В кн.: VIII Международная конференция по математическому моделированию: тезисы докладов.: Якутск: Северо-Восточный федеральный университет им. М.К. Аммосова, 2017. С. 108–109.

Методом молекулярной динамики (МД) проведен компьютерный анализ структурно-динамических параметров ряда трансмембранных (ТМ) α-спиральных сегментов белков, встроенных в гидратированные липидные бислои различного химического состава. В качестве объектов исследования выбраны мономеры ТМ спиралей нескольких рецепторных тирозинкиназ, отвечающих за клеточную сигнализацию, а также образуемые ими гомо- и гетеро-димеры. Установлено, что в ходе МД все типы ТМ-спиралей в целом ...

Added: November 14, 2017

Выявление молекулярных основ взаимодействия белков в мембране с помощью компьютерного моделирования

Kuznetsov A., Efremov R., В кн.: Физико-химические механизмы и регуляция процессов трансформации энергии в биологических структурах.: Ижевск: Ижевский институт компьютерных исследований, 2017. Гл. 1.1 С. 9–32.

В главе 1.1 (Кузнецов, Ефремов) рассмотрены теоретические подходы к изучению мембранных белков (МБ), которые являются важнейшим компонентом плазматической мембраны клетки. Они выполняют жизненно важные функции, а нарушения в их работе приводят к развитию патологических состояний. Изучение МБ экспериментальными методами требует особых подходов и техник, поскольку для их функционирования необходимо сохранение мембранного окружения. Поэтому несмотря на ...

Added: November 14, 2017

Why human anti-Galα1–4Galβ1–4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane? Molecular dynamics and immunochemical investigation

Volynsky P., Efremov R., Mikhalev I. et al., Molecular Immunology 2017 Vol. 90 P. 87–97

Background Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration. Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk) practically in all humans. Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) – normal component of erythrocyte and endothelial cell membrane, i.e. the ...

Added: November 13, 2017

Lipid environment promotes dimerization of transmembrane helices

Kuznetsov A., Polyansky A., Volynsky P. et al., , in: Computational Materials and Biological Sciences.: [б.и.], 2015. P. 139–150.

Membrane receptors are among the most important molecules in the cell. Their functioning determines the response of the cell to the external conditions. Also, they provide cellular communication and recognitionmechanisms. So, their proper functioning is a vital question of life. There is a lot of data today about these receptors. Many three-dimensional (3D) structures are ...

Added: May 12, 2016

Компартментальное моделирование транспортных процессов в корне растения, учитывающее присутствие пограничных слоев

Logvenkov S. A., Штейн А. А., Российский журнал биомеханики 2013 Т. 17 № 4 С. 37–46

Basing on the information about the structure of the solution and asymptotic estimates in the problem of steady flow across the root, a system of algebraic relations similar to the commonly used compartment models is obtained. As compared with these, the method proposed has an important advantage making it possible to take into account the ...

Added: January 30, 2014