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Роль мембранного окружения в процессе димеризации трансмембранных сегментов рецепторных тирозинкиназ семейства ErbB
С. 165–165.
Kuznetsov A., Efremov R.
In book
Т. 1. , Краснодар: Полиграфическое объединение «Плехановец», 2019.
Volynsky P., Urban A., Pavlov K. et al., International Journal of Molecular Sciences 2025 Vol. 26 No. 2 Article 553
Alzheimer’s disease (AD) pathogenesis is correlated with the membrane content
of various lipid species, including cholesterol, whose interactions with amyloid precursor
protein (APP) have been extensively explored. Amyloid-β peptides triggering AD are
products of APP cleavage by secretases, which differ depending on the APP and secretase location
relative to ordered or disordered membrane microdomains. We used high-resolution
NMR to probe ...
Added: October 12, 2025
Goryashchenko A., Mozhaev AA, Serova O. et al., Biomolecules 2020 Vol. 10 No. 7 P. 1060–0
To study the structure and function of the pH-regulated receptor tyrosine kinase insulin receptor-related receptor (IRR), а member of the insulin receptor family, we obtained six mouse monoclonal antibodies against the recombinant IRR ectodomain. These antibodies were characterized in experiments with exogenously expressed full-length IRR by Western blotting, immunoprecipitation, and immunocytochemistry analyses. Utilizing a previously ...
Added: February 4, 2024
Bershatsky Y., Kuznetsov A., Idiatullina A. et al., International Journal of Molecular Sciences 2023 Vol. 24 No. 4 Article 3906
Human InsR, IGF1R, and IRR receptor tyrosine kinases (RTK) of the insulin receptor
subfamily play an important role in signaling pathways for a wide range of physiological processes
and are directly associated with many pathologies, including neurodegenerative diseases. The
disulfide-linked dimeric structure of these receptors is unique among RTKs. Sharing high sequence
and structure homology, the receptors differ dramatically ...
Added: May 19, 2023
Aliper E. T., Krylov Nikolay A., Nolde Dmitry E. et al., International Journal of Molecular Sciences 2022 Vol. 23(16) Article 9221
Understanding fusion mechanisms employed by SARS-CoV-2 spike protein entails realistic
transmembrane domain (TMD) models, while no reliable approaches towards predicting the 3D
structure of transmembrane (TM) trimers exist. Here, we propose a comprehensive computational
framework to model the spike TMD only based on its primary structure. We performed amino acid
sequence pattern matching and compared the molecular hydrophobicity potential ...
Added: August 25, 2022
Eliseev I. E., Yudenko A. N., Vysochinskaya V. V. et al., F1000Research 2018 Vol. 7 Article 57
Background: The ability of ErbB3 receptor to functionally complement ErbB1-2 and induce tumor resistance to their inhibitors makes it a unique target in cancer therapy by monoclonal antibodies. Here we report the expression, purification and structural analysis of a new anti-ErbB3 single-chain antibody.
Methods: The VHH fragment of the antibody was expressed in E. coli SHuffle cells as ...
Added: September 24, 2021
Kuznetsov A., Bennasroune A., Maurice P. et al., FEBS Open Bio 2021 Vol. 11 No. Suppl.1 P. 219–219
The receptor tyrosine kinase (RTK) superfamily comprises many different cell-surface receptors having similar membrane organization and function with signal transduction occuring in the dimeric state. Insulin receptor (IR) and type 1 insulin-like growth factor receptor (IGF1R) differ from other RTKs being constitutively homodimeric transmembrane glycoproteins, and molecular mechanisms of their activation still remain elusive. Current ...
Added: August 30, 2021
Albrecht C., Kuznetsov A., Appert-Collin A. et al., Frontiers in Cell and Developmental Biology 2020 Vol. 8 P. 611121
Sialidases, or neuraminidases, are involved in several human disorders such as neurodegenerative, infectious and cardiovascular diseases, and cancers. Accumulative data have shown that inhibition of neuraminidases, such as NEU1 sialidase, may be a promising pharmacological target, and selective inhibitors of NEU1 are therefore needed to better understand the biological functions of this sialidase. In the ...
Added: December 14, 2020
Kuznetsov A., Efremov R., В кн.: Математика. Компьютер. Образование. Тезисы.Вып. 27: Двадцать седьмая международная конференция "Математика. Компьютер. Образование." Симпозиум с международным участием "Биофизика сложных систем, вычислительная и системная биология, молекулярное моделирование".: Ижевск: Ижевский институт компьютерных исследований, 2020. С. 53–54.
Receptor tyrosine kinases form an important class of membrane proteins that regulate cell life cycle and metabolism. Among wide variety of receptors, they have simple structure with single alpha-helix crossing the membrane. This transmembrane domain is functionalm as dimeric state conformation defines active/inactive state of the receptor. The lipid environment can modulate functioning of such ...
Added: February 3, 2020
Kuznetsov A., Bennasroune A., Maurice P. et al., В кн.: II объединенный научный форум: VI съезд физиологов СНГ, VI съезд биохимиков России, IX российский симпозиум «Белки и пептиды» (Сочи, Дагомыс, 1–6 октября 2019). Научные труды.Т. 2.: М.: Издательство "Перо", 2019. С. 108–108.
Нейраминидаза-1 (NEU1) – фермент, катализирующий отщепление терминальных остатков сиаловых кислот от олигосахаридов. В клетках человека NEU1 представлена в двух формах: лизосомальной и мембраносвязанной. Структурные особенности последней и её функция в составе комплекса рецептора эластина (ERC) не до конца ясны. Доказано, что в мембране происходит димеризация NEU1, а мутации в последовательности второго трансмембранного домена (ТМ2) NEU1 ...
Added: November 2, 2019
Kuznetsov A., Efremov R., В кн.: Тезисы докладов XXVI Международной конференции "Математика. Компьютер. Образование". МКО-2019.: [б.и.], 2019. С. 45–45.
Transmembrane (TM) helices are one of the most common structural elements in membrane proteins. Their interaction in lipid environment governs the formation of protein spatial structure formation and functioning in normal and pathology. Receptor tyrosine kinases are a wide class of protein molecules having single helix as their TM domain, so dimerization process is crucial ...
Added: November 1, 2019
Urban A., Bocharov E., Nadezhdin K. et al., , in: FEBS Open Bio Volume 9, Issue S1 Supplement: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019Vol. 9: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019.: Oxford: Wiley, 2019. Ch. 31-016 P. 299–299.
Bitopic proteins having only one helical transmembrane (TM) domain are a class of biologically significant membrane proteins, including the type I receptors and amyloid precursor protein (APP), which are involved in regulating the homeostasis of human organism and recognized as substrate by c-secretase. Amyloid Ab-peptides forming plaques in brain during Alzheimer disease (AD) are the ...
Added: November 1, 2019
Kuznetsov A., Maurice P., Efremov R., , in: FEBS Open Bio Volume 9, Issue S1 Supplement: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019Vol. 9: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019.: Oxford: Wiley, 2019. Ch. 27-068 P. 268–269.
Receptor tyrosine kinases (RTK) are vital players in cell signaling governing growth and proliferation. These integral membrane proteins work only in dimeric states, so the conformation of transmembrane dimer determines the signal transferred into cell. Here, we used modern molecular modeling techniques to study details of protein-protein and protein-lipid interactions in model systems containing monomers ...
Added: November 1, 2019
Zamaletdinov M., Kuznetsov A., Maurice P. et al., , in: FEBS Open Bio Volume 9, Issue S1 Supplement: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019Vol. 9: 44th FEBS Congress, From Molecules to Living Systems, Krakow, Poland, July 6‐11, 2019.: Oxford: Wiley, 2019. Ch. 27-047 P. 262–262.
Insulin receptor (IR) family is represented by three membrane proteins participating in organism development, growth, and vital activity. Modulation of the functioning of these receptors by external agents looks very perspective from a pharmaceutical point of view. Although IR is well studied, little is known about the role of its transmembrane (TM) domain in receptor ...
Added: November 1, 2019