• A
  • A
  • A
  • ABC
  • ABC
  • ABC
  • А
  • А
  • А
  • А
  • А
Regular version of the site
Of all publications in the section: 9
Sort:
by name
by year
Article
Ugrumov M. Doklady Biochemistry and Biophysics. 2020. Vol. 490. P. 34-37.

One of the features of the developing suprachiasmatic nucleus (SCN), the “biological clock” of the body, is the early expression of dopamine (DA) receptors in the absence of dopaminergic neurons as a source of DA. Only recently we showed that DA in SCN is synthesized together by nerve fibers containing only tyrosine hydroxylase (TH) and neurons containing only aromatic L-amino acid decarboxylase (AADC). This study was aimed to assess specific characteristics of the phenotype of TH-fibers in ontogenesis. For this purpose, PCR and immunohistochemical analysis of the expression of genes and proteins such as TH, AADC, vesicular monoamine transporter (VMAT), and receptors for DA (D1, D2) was performed. We have detected numerous TH-immunoreactive fibers in SCN of young and adult rats. VMAT was observed in some of them, which suggests vesicular storage of L-DOPA. Considering the key role of TH-fibers in cooperative synthesis of DA, we assumed the presence of their dopamine regulation. Using double immunolabeling, we showed that D1 and D2 are present in TH-fibers in adult rats, and only D1 in young rats. According to PCR, D1 and D2 are also expressed in neurons of SCN in adult rats and only D1 in young rats. Thus, it was shown for the first time that VMAT and D1 are coexpressed in TH-fibers synthesizing L-DOPA in SCN in young and adult rats, and also D2 receptors in adult rats, which suggests vesicular storage and dopamine regulation of L-DOPA secretion, respectively.

Added: Dec 6, 2020
Article
Knyazev E. N., Paul S. Yu., Tonevitsky A. G. Doklady Biochemistry and Biophysics. 2021. Vol. 499. No. 1. P. 251-256.

Choriocarcinoma cells BeWo b30 are used to model human placental trophoblast hypoxia using cobalt (II) chloride and hydroxyquinoline derivative (HD) as chemical inducers of hypoxia-inducible factor (HIF). In this study, it was shown that both substances activate the hypoxic pathway and the epithelial–mesenchymal transition and inhibit the pathways of cell proliferation. However, CoCl2 caused activation of the apoptosis pathway, increased the activity of effector caspases 3 and 7, and increased the expression of the unfolded protein response target DDIT3. The mTORC1 pathway was activated upon exposition to CoCl2, while HD suppressed this pathway, as it happens during real trophoblast hypoxia. Thus, effect of CoCl2 on BeWo cells can be a model of severe hypoxia with activation of apoptosis, while HD mimics moderate hypoxia.

Added: Aug 26, 2021
Article
Shkurnikov M. Yu., Nersisyan S. A., Osepyan A. S. et al. Doklady Biochemistry and Biophysics. 2020. Vol. 493. No. 1. P. 208-210.

Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change intracellular regulatory mechanisms. In the framework of this study, it was shown that the quantitative estimates of inaccuracies in Drosha and Dicer activity significantly differ between the specimens of normal colon tissue and malignant colorectal tumors.

Added: Oct 13, 2020
Article
Syomin B., Pelisson A., Ilyin Y. et al. Doklady Biochemistry and Biophysics. 2004. Vol. 398. P. 310-312.

Expression of the retrovirus Gypsy Gag in Spodoptera frugiperda cell culture with the recombinant baculovirus

Added: May 18, 2017
Article
S. A. Nersisyan, M. Yu. Shkurnikov, E. N. Knyazev. Doklady Biochemistry and Biophysics. 2020. Vol. 493. No. 1. P. 205-207.

One of the main complications of pregnancy and causes of maternal and perinatal mortality is preeclampsia. The pathophysiology of preeclampsia is associated with the development of placenta and fetal hypoxia and secretion of a number of effective molecules. The human choriocarcinoma cell line BeWo b30 is often used as a model of the placental barrier. It was shown that oxyquinoline derivatives can mimic hypoxia by suppressing HIF-prolyl hydroxylases and the accumulation of HIF-1α. This effect also leads to a change in the expression of microRNAs and their target genes. However, with hypoxia in cells, not only the level of individual miRNAs but also the ratio of miRNA isoforms (isomiRs) can change, presumably due to inaccuracies in the work of the Drosha and Dicer enzymes. In this work, we showed a change in the expression of the factors involved in the maturation of miRNAs when simulating hypoxia in BeWo b30 cells with an oxyquinoline derivative, which may be one of the causes for the change in the ratio of miRNA isoforms.

Added: Oct 13, 2020
Article
Maltseva D. V., Raigorodskaya M. P., Zgoda V. G. et al. Doklady Biochemistry and Biophysics. 2020. Vol. 494. No. 1. P. 219-221.

In the present study, we assessed the role of annexin 13 membrane-binding protein (ANXA13) in the intracellular transport of vesicles containing type II ribosome-inactivating proteins (RIP-IIs). A modified human intestinal epithelial cell line HT29 was used, in which the expression of ANXA13 was significantly reduced. The cytotoxic effect of ricin and viscumin was evaluated by modification of 28S ribosome RNA. The observed differences in the activity of toxins on the parental and modified HT29 lines indicate that ANXA13 plays a different role in the intracellular transport of vesicles containing the RIP-IIs.

Added: Feb 1, 2021
Article
Bondarenko N. S., Murtazina A. R., Nikishina Y. O. et al. Doklady Biochemistry and Biophysics. 2017. Vol. 472. No. 1. P. 23-26.

The level of gene expression and the protein content of tyrosine hydroxylase and dopamine β-hydroxylase were determined. In the perinatal period of rats, when noradrenaline functions as a morphogenetic factor, the level of gene expression of these enzymes increased and the content of protein products of these genes was almost unchanged, indicating the difference in the regulatory mechanisms of their transcription and translation.

Original Russian Text © N.S. Bondarenko, A.R. Murtazina, Yu.O. Nikishina, A.Ya. Sapronova, M.V. Ugrumov, 2017, published in Doklady Akademii Nauk, 2017, Vol. 472, No. 3, pp. 341–344.

 

 

Added: Jun 8, 2017
Article
Maltseva D. V., Raigorodskaya M. P., Tikhonova O. V. et al. Doklady Biochemistry and Biophysics. 2020. Vol. 493. No. 1. P. 198-200.

The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated ribosomes, it was shown that the contribution of the proteasome to the degradation of the catalytic subunits of toxins is different. The role of the Cdc37 co-chaperone in maintaining the stability of A subunit of viscumin in the cytoplasm is shown.

Added: Feb 1, 2021
Article
Bondarenko N., Murtazina A., Dil’mukhametova L. et al. Doklady Biochemistry and Biophysics. 2016. Vol. 467. No. 1. P. 153-156.

Spontaneous and K+-stimulated release of noradrenaline from the hypothalamus, adrenal gland, and organ of Zuckerkandl under their flowing incubation was investigated in the perinatal period of ontogenesis of rats. The results suggest that, during the investigated period of ontogenesis, adrenal glands are the main source of noradrenaline in the blood, whereas the contributions of the organ of Zuckerkandl and the brain are not as significant and change during this period. © 2016, Pleiades Publishing, Ltd.

Added: Jul 8, 2016