?
Alteration in the Expression of Genes Encoding Primary Metabolism Enzymes and Plastid Transporters during the Culture Growth of Chlamydomonas reinhardtii
Molecular Biology. 2020. Vol. 54. No. 4. P. 503–519.
Puzanskiy R. K., Romanyuk D. A., Kirpichnikova A. A., Shishova M.
In a mixotrophic Chlamydomonas reinhardtii culture, the expression levels of genes encoding primary metabolic enzymes and chloroplast plastid transporters were analyzed. For the majority of the genes studied, their expression levels decreased as they approached the final stages of culture growth. During the period of exponential growth, the expression profiles changed more intensively than during the stationary phase. In the middle of exponential growth, significant changes of mRNA profiles reflected reorganization of metabolism, with an emphasis on the induction of lipid synthesis, accompanied by alterations in carbon fluxes through biochemical pathways and a shift in the energy balance between the plastid and cytosol.
Forysenkova Anna A., Konovalova M., Fadeeva I. et al., Materials 2023 Vol. 16 No. 16 P. 5532–5549
Surgical operations on the peritoneum are often associated with the formation of adhesions, which can interfere with the normal functioning of the internal organs. The effectiveness of existing barrier materials is relatively low. In this work, the effectiveness of soluble alginate–polyvinylpyrrolidone (PVP-Alg) and non-soluble Ca ion cross-linked (PVP-Alg-Ca) films in preventing these adhesions was evaluated. ...
Added: August 12, 2025
Dzhalilova D., Silina M., Tsvetkov I. et al., International Journal of Molecular Sciences 2024 Vol. 25 No. 14 Article 7801
One of the factors contributing to colorectal cancer (CRC) development is inflammation, which is mostly hypoxia-associated. This study aimed to characterize the morphological and molecular biological features of colon tumors in mice that were tolerant and susceptible to hypoxia based on colitis-associated CRC (CAC). Hypoxia tolerance was assessed through a gasping time evaluation in a ...
Added: March 25, 2025
Blokhin V., Shupik M., Gutner U. et al., Biomolecules 2022 Vol. 12 No. 1 Article 93
Parkinson’s disease (PD) is a neurodegenerative disease incurable due to late diagnosis and treatment. Therefore, one of the priorities of neurology is to study the mechanisms of PD pathogenesis at the preclinical and early clinical stages. Given the important role of sphingolipids in the pathogenesis of neurodegenerative diseases, we aimed to analyze the gene expression ...
Added: March 4, 2024
Kolacheva A., Pavlova E., Bannikova A. et al., International Journal of Molecular Sciences 2023 Vol. 24 No. 3 Article 3027
Given the limited access to clinical material for studying the pathogenesis of Parkinson's disease (PD), these studies should be carried out on experimental models. We have recently developed a subchronic model of the progressive development of PD with a gradual transition from the preclinical (asymptomatic) stage to the clinical (symptomatic) one. The aim of this ...
Added: March 1, 2024
Ivanov K., Samuilova O., Zamyatnin A., Cellular and Molecular Life Sciences 2023 Vol. 80 No. 8 Article 197
Recent advances in RNA sequencing technologies helped uncover what was once uncharted territory in the human genome—the complex and versatile world of long noncoding RNAs (lncRNAs). Previously thought of as merely transcriptional “noise”, lncRNAs have now emerged as essential regulators of gene expression networks controlling development, homeostasis and disease progression. The regulatory functions of lncRNAs ...
Added: December 11, 2023
Herbert A., Pavlov F., Dmitrii Konovalov et al., International Journal of Molecular Sciences 2023 Vol. 24 No. 5 Article 4884
The classical view of gene regulation draws from prokaryotic models, where responses to environmental changes involve operons regulated by sequence-specific protein interactions with DNA, although it is now known that operons are also modulated by small RNAs. In eukaryotes, pathways based on microRNAs (miR) regulate the readout of genomic information from transcripts, while alternative nucleic ...
Added: March 17, 2023
Granata I., Manipur I., Giordano M. et al., Scientific data 2022 Vol. 9 Article 607
Studies about the metabolic alterations during tumorigenesis have increased our knowledge of the underlying mechanisms and consequences, which are important for diagnostic and therapeutic investigations. In this scenario and in the era of systems biology, metabolic networks have become a powerful tool to unravel the complexity of the cancer metabolic machinery and the heterogeneity of ...
Added: October 30, 2022
Loginov V., Pronina I., Filippova E. et al., International Journal of Molecular Sciences 2022 Vol. 23 No. 3 Article 1300
Our work aimed to differentiate 20 aberrantly methylated miRNA genes that participate at different stages of development and metastasis of ovarian carcinoma (OvCa) using methylation-specific qPCR in a representative set of clinical samples: 102 primary tumors without and with metastases (to lymph nodes, peritoneum, or distant organs) and 30 peritoneal macroscopic metastases (PMM). Thirteen miRNA ...
Added: June 20, 2022
Хушпульян Д. М., Nikulin S., Христиченко А. Ю. et al., Вестник Московского университета. Серия 2: Химия 2021 Т. 62 № 3 С. 213–222
The attempts to elucidate the mechanism of hypoxic activation of erythropoietin synthesis let to the
discovery of hypoxia inducible factor (HIF) and its stability regulation via HIF prolyl hydroxylase, iron
and α-ketoglutarate dependent dioxygenase. Enzyme inhibitors mimic the action of hypoxia, however, are
far more specific. Comparative transcriptomic microanalysis of various types on the enzyme inhibitors
with hypoxia at ...
Added: December 20, 2021
Nersisyan S., Galatenko A. V., Ahlers A. et al., Biochimie 2022 Vol. 192 P. 91–101
In this study we analyzed expression of CD24 in a cohort of colorectal cancer patients using immunohistochemistry staining of CD24. We found a significant association between absence or low expression of CD24 (10% of membranous and 55% of cytoplasmic staining) and shortened patient survival. Protein localization played a crucial role in the prognosis: membranous form ...
Added: October 23, 2021
Puzanskiy R. K., Romanyuk D. A., Shishova M., Russian Journal of Plant Physiology 2020 Vol. 67 No. 5 P. 867–878
Chlamydomonas reinhardtii P.A. Dangeard is a unicellular green alga capable to assimilate acetate. C. reinhardtii growth and metabolism distinctly depend on trophic conditions. Its influence on batch culture interferes with changes in the medium composition and metabolism of microalgae occurring during culture growth. The aim of this work was to estimate the effect of acetate on changes in ...
Added: October 22, 2020
Елисютина О. Г., Болдырева М. Н., Rebrova O. et al., Российский аллергологический журнал 2018 Т. 15 № 6 С. 33–44
The basis for the development of atopic dermatitis (AD) is genetic predisposition, hypersensitivity to allergens, Th1/Th2 disbalance, increased degranulation of mast cells and antigen-presenting activity of Langerhans cells, as well as epidermal barrier dysfunction. Recently, genotypes, phenotypes and endotypes of AD, and biomarkers, which can be used to assess the effectiveness of therapy and to ...
Added: October 23, 2019
Mazin P., Nature 2019 Vol. 571 P. 505–509
The evolution of gene expression in mammalian organ development remains largely uncharacterized. Here we report the transcriptomes of seven organs (cerebrum, cerebellum, heart, kidney, liver, ovary and testis) across developmental time points from early organogenesis to adulthood for human, rhesus macaque, mouse, rat, rabbit, opossum and chicken. Comparisons of gene expression patterns identified correspondences of developmental ...
Added: August 28, 2019
Alexandrov Yuri I., Sozinov A., Svarnik O. et al., Advances in Neurobiology 2018 Vol. 21 P. 1–33
Despite the years of studies in the field of systems neuroscience, functions of neural circuits and behavior-related systems are still not entirely clear. The systems description of brain activity has recently been associated with cognitive concepts, e.g. a cognitive map, reconstructed via place-cell activity analysis and the like, and a cognitive schema, modeled in consolidation ...
Added: November 13, 2018
Tonevitsky A., Kononenko O., Mityakina I. et al., Brain Research 2018 Vol. 1695 P. 78–83
The endogenous opioid system (EOS) controls the processing of nociceptive stimuli and is a pharmacological target for opioids. Alterations in expression of the EOS genes under neuropathic pain condition may account for low efficacy of opioid drugs. We here examined whether EOS expression patterns are altered in the lumbar spinal cord of the rats with ...
Added: October 30, 2018
Popov D., Lysenko E., Bokov R. et al., Physiological Reports 2018 No. Sep;6(17):e13868 P. 1–13
Most studies examining the molecular mechanisms underlying adaptation of human skeletal muscles to aerobic exercise focused on the response to acute exercise. Here, we examined the effect of a 2-month aerobic training program on baseline parameters in human muscle. Ten untrained males performed a one-legged knee extension exercise for 1 h with the same relative intensity ...
Added: October 25, 2018
Mathe C., Peresetsky A., Dehais P. et al., Journal of Molecular Biology 1999 Vol. 285 No. 5 P. 1977–1991
While genomic sequences are accumulating, finding the location of the genes remains a major issue that can be solved only for about a half of them by homology searches. Prediction methods are thus required, but unfortunately are not fully satisfying. Most prediction methods implicitly assume a unique model for genes. This is an oversimplification as ...
Added: April 16, 2018
Александров Ю.И., Сварник О. Е., Знаменская И. И. et al., Вопросы психологии 2017 № 3 С. 80–91
Alcoholism as a cause of regression in learning and in pro-social behavior
Alexandrov Yu.I., Svarnik O.Ye., Znamenskaya I.I., Arutiunova K.R., Kolbeneva M.G., Krylov A.K., Bulava A.N.
Despite a long history of the notion of regression, its manifestations and mechanisms are still poorly researched, specific experimental studies are rare, points of view conflict. A study was conducted to discover dynamics of subjective experience and brain activity lying ...
Added: January 30, 2018
Bondarenko N. S., Murtazina A. R., Nikishina Y. O. et al., Doklady Biochemistry and Biophysics 2017 Vol. 472 No. 1 P. 23–26
The level of gene expression and the protein content of tyrosine hydroxylase and dopamine β-hydroxylase were determined. In the perinatal period of rats, when noradrenaline functions as a morphogenetic factor, the level of gene expression of these enzymes increased and the content of protein products of these genes was almost unchanged, indicating the difference in ...
Added: June 8, 2017
Sakharov D. A., Maltseva D. V., Riabenko Evgeniy A et al., European Journal of Applied Physiology 2012 Vol. 112 No. 3 P. 963–972
High and moderate intensity endurance exercise alters gene expression in human white blood cells (WBCs), but the understanding of how this effect occurs is limited. To increase our knowledge of the nature of this process, we investigated the effects of passing the anaerobic threshold (AnT) on the gene expression profile in WBCs of athletes. Nineteen ...
Added: October 14, 2016
Maltseva D. V., Khaustova N. A., Fedotov N. N. et al., Journal of Clinical Bioinformatics 2013 Vol. 3 No. 1
Background
Quantification and normalization of RT-qPCR data critically depends on the expression of so called reference genes. Our goal was to develop a strategy for the selection of reference genes that utilizes microarray data analysis and combines known approaches for gene stability evaluation and to select a set of appropriate reference genes for research and clinical ...
Added: October 30, 2015
Shkurnikov M. Y., V. V. Galatenko, Lebedev A. E. et al., Bulletin of Experimental Biology and Medicine 2014 Vol. 157 No. 4 P. 454–458
The search for novel parameters to predict the risk of relapse in breast cancer was conducted. Significant correlation between the risk of relapse and α-2A adrenergic receptor (ADRA2A) expression was revealed using public microarray datasets. This relationship was confirmed by validation on independent microarray dataset. It was found that when assessing the risk of BC ...
Added: October 28, 2015