Сравнение экспрессии маркеров эпителиально-мезенхимальной трансформации в 2D- и 3D-культурах клеток колоректального рака в присутствии ламининов 332 и 411

Молекулярная биология. 2019. Т. 53. № 2. С. 330–338.

Мальцева Д. В., Макарова Ю. А., Хритиченко А. Ю., Цыпина И. М., Тоневицкий Е. А., Родин С. А.

The necessary stage of epithelial-mesenchymal transformation (EMT) is the loss of apical-basal polarization by cells, in the formation of which an important role is played by the interaction of cells with the basement membrane and its laminins. The effect of the transition of colorectal cancer cell lines from the polarized state (2D) to the absence of apical-basal polarization (3D) on gene expression of epithelial and mesenchymal markers was investigated, and the role of laminins 332 and 411 (LM-332 and LM-411, respectively) in this process. The three studied cell lines, HT-29, HCT-116, and RKO, were found to exhibit different sensitivity to changes in cultivation conditions (2D / 3D) and the presence of laminins. One reason for this may be a difference in the initial state of the cells. It was shown that in the initial, 2D state, the lines under investigation were at different stages of EMT. In the HT-29 line, the expression profile characteristic of epithelial cells prevailed, in RKO, for mesenchymal, and in HCT-116, for the intermediate state. The most sensitive to the effects was the cell line HCT-116. No critical rearrangement of the profile of EMT genes was observed either during the transition from 2D to 3D cultivation or when laminins were added. However, for both effects, changes in the expression of the SNAI1 and ZEB1 genes, which encode transcription factors regulating the process of EMT, were recorded; moreover, the expression of SNAI1 increased in response to treatment with laminin in all three cell lines.

Keywords: базальная мембрана, колоректальный рак, HT-29, HCT-116, RKO, эпителиально-мезенхимальная трансформация, апикально-базальная поляризация, in vitro модель, 2D-культура, 3D-культура, ламинин-332, ламинин-5, ламинин-411, ламинин-8